以黄连素/羟丙基-β-环糊精为荧光探针检测金刚烷胺

Determination of Amantadine Using Complexation of Berberine and Hydroxypropyl-β-cyclodextrin

为了建立一种金刚烷胺(AMD)检测的新方法,以盐酸黄连素/羟丙基-P-环糊精(BRH/HP-β-CD)为荧光探针,将HP-β-CD分别与BRH、AMD相互作用,测定AMD、BRH/HP-β-CD及两者结合的荧光强度变化规律,并验证分析该荧光分光光度法的适用性。结果表明,HP-β-CD与BRH生成1∶1的包合物并显著增强黄连素水溶液的荧光强度,当加入AMD后,BRH/HP-β-CD包合物的荧光强度逐渐减弱,据此成功建立一种以BRH/HP-β-CD为荧光探针的金刚烷胺检测新方法。当AMD溶液浓度在0.05～4.5 mg·L^(-1)范围内时与荧光猝灭值△F之间呈线性关系,相关系数为0.989 3,检测限(S/N=3)为0.03 mg·L^(-1)。溶液pH值和常见的药物赋形剂均不会对测量结果造成干扰,将本方法用于盐酸金刚烷胺药片的分析,其回收率在92%～101%范围内,相对标准偏差小于1%,说明该方法能够成功用于金刚烷胺的测定。本研究为以超分子包合物为荧光探针用于金刚烷胺类药物的检测提供了理论依据。

In order to develop a new method for the determination of amantadine(AMD),Berberine hydrochloride/hydroxypropyl-β-cyclodextrin(BRH/HP-β-CD)was used as a fluorescent probe,HP-β-CD was interacted with BRH and amantadine,respectively.The interaction of AMD,BRH/HP-β-CD was determined and the applicability of the fluorescence spectrophotometry was verified.The results showed that HP-β-CD and BRH formed a 1∶1 inclusion complex and the fluorescence intensity increased significantly.The fluorescence intensity of the berberine/hydroxypropyl-β-cyclodextrin inclusion complex gradually decreased,after adding amantadine to the solution,which can help to establish a new method for the detection of amantadine with BRH/HP-β-CD as fluorescent probe.When the concentration of amantadine solution was within the range of 0.05~4.5 mg·L-1,it had a linear relationship with the relative fluorescence intensity.The correlation coefficient was 0.9893,and the detection limit(S/N=3)was 0.03 mg·L-1.Both pH value and common pharmaceutical excipients had little effect of the results.The recovery of this method ranged from 92%to 101%with relative standard deviations less than 1%,indicating that this method can be successfully applied to the determination of amantadine.This study provided a theoretical basis for the determination of amantadine drugs using supramolecular inclusion complex as fluorescent probe.