干旱胁迫下野生大豆ABC转运蛋白转录组测序分析

Sequence Analysis of ABC Transporter Transcriptome in Wild Soybean Under the Drought Stress

为获取干旱胁迫状态下ABC转运蛋白家族相关基因,以抗旱性强的野生大豆为试验材料,浇灌20%PEG 6000模拟干旱胁迫,分别在干旱处理0、6、12、24和48 h后取叶片提取RNA进行转录组测序;以GO、KEGG和NR三大数据库注释的结果为基础,再以ABC转运蛋白为关键词进行筛选。结果表明,共获得337条ABC转运蛋白相关序列,包括8大亚家族(ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG和ABCI)。差异表达分析结果表明,共有182条表现差异表达。GO注释到的差异表达基因多数为嘌呤核苷酸分解过程,KEGG注释到的差异表达基因分布在5个亚家族(ABCA、ABCB、ABCC、ABCD和ABCG)。对NR数据库有注释的168条差异表达基因进行进化分析,结果表明ABCE、ABCF、ABCG家族与ABCI11为一类,ABCB、ABCC家族与ABCI10为一类,ABCA、ABCD家族与ABCI19为一类。本研究为进一步研究野生大豆干旱胁迫下ABC转运蛋白抗旱基因提供了一定的理论依据。

In order to obtain the genes related to ABC transporter protein family under the conditions of drought stress,the drought-resistant wild soybean accession was used as experimental material which was treated with 20%PEG6000 solution to simulate drought stress.RNA was extracted from leaves of drought treated 0,6,12,24 and 48 h respectively,and transcriptome sequencing was carried out by Illumina Hi Seq 2000 platform.Based on the results of GO,KEGG and NR 3 major database notes,and then using ABC transporter as the key words to screen,a total of 337 ABC transporter related sequences were obtained,including 8 subfamilies(ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG和ABCI).Differential expression analysis showed that there were 182 differentially expressed genes.Most of the differentially expressed genes annotated by GO database were purine ribonucleotide catabolic processes,while the Unigenes annotated by KEGG database were distributed in the 5 subfamilies(ABCA、ABCB、ABCC、ABCD、ABCE、ABCF、ABCG和ABCI).The evolutionary analysis of 168 differentially Unigenes annotated in the NR database shows that the ABCE,ABCF,ABCG families and ABCI11 were clustered to a group,the ABCB,ABCC families and ABCI10 were clustered to a group,and the ABCA,ABCD families and ABCI19 were clustered to a group.The information obtained from ABC transporters provides a theoretical basis for further study on drought resistance genes of ABC transporters in wild soybean.