福建漳浦野生稻稻瘟病抗性鉴定评价和抗性基因检测

Identification and Gene Detection of Blast Resistance for Zhangpu Wild Rice(Oryza rufipogon Griff.)in Fujian Province

为挖掘和利用福建漳浦普通野生稻稻瘟病抗性资源,采用自然诱发对福建漳浦普通野生稻2个居群(石湖潭和古糖)67份材料的苗瘟、叶瘟和穗瘟进行抗性鉴定评价,并用Pi9、Pid2、Pi5、Pi2、Pi54和Pikm共6个抗性基因的功能标记分析基因型。结果表明,不同时期病级差异明显,变异系数在31.65%~43.46%之间,苗期平均病级为7.51,分糵期和成熟期平均病级分别为5.15和5.84,2个自然居群3个时期的稻瘟病抗性差异不显著,筛选出M1044、M2010和M2016共3份表现中抗(3级)的材料。漳浦野生稻稻瘟病总体抗性差,易感稻瘟病。苗瘟与叶瘟呈极显著正相关,相关系数为0.82;穗颈瘟与苗瘟和叶瘟呈极显著正相关,相关系数分别为0.33和0.41。67份材料中,4份含有稻瘟病Pi9抗性基因、17份含有Pi5抗性基因、23份含有Pi54抗性基因、2份含有Pikm抗性基因,均不含Pi2、Pid2这2个抗性基因;材料含有的抗性基因数量在0~3个之间,其中有30份材料不含所检测的抗性基因,29份材料含1个抗性基因,7份材料含2个抗性基因,1份材料含3个抗性基因。本研究结果为漳浦野生稻资源的深入挖掘和利用提供了一定的理论参考。

In order to explore and utilize rice resistant blast germplasms,a total of 67 Zhangpu wild rices from 2 natural populations in Fujian Province were assessed for blast resistance of seedling,leaf and panicle by natural infection,and the resistant blast genotypesby were detected functional molecular markers of resistance genes Pi9,Pid2,Pi5,Pi2,Pi54 and Pikm.The results showed that there was a obvious difference of blast disease grade in various growth stages,and the coefficient of variation ranged from 31.65%to 43.46%.The average blast disease grade was 7.51,5.15 and 5.84 at seedling,tillering and maturing stage,respectively.The blast resistance for three various stages showed no significant difference between 2 natural populations from Shihutan and Gutang.M1044,M2010 and M2016 exhibited moderate resistance(three grade),indicating that Zhangpu wild rice had a poor resistance and was susceptible to blast.The correlation analysis indicated that there was a closely significant positive correlation between seedling and leaf blast,with the correlation coefficient of 0.82.Panicle neck blast was highly significantly correlated with both seedling and leaf blast,and the correlation coefficients were 0.33 and 0.41,respectively.Among 67 Zhangpu wild rice materials,4 rice accessions owned Pi9,17 accessions have PiS,23 accessions have Pi54 and 2 materials have Pik.None had both Pi2 and Pid2 resistant genes.The number of resistant genes in these materials ranged from 0 to 3,among which 30 materials did not contain the tested resistance genes,29 rice materials contained 1 resistance gene,7 materials had 2 resistance genes and 1 accesion contained 3 resistance genes.These results can provide a theoretical reference for further excavation and utilization of Zhangpu wild rice germplasms.