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不同耐旱性甘薯柴根和块根的差异蛋白组分析 被引量:1

Analysis of Differential Proteome in Thick Root and Storage Root of Sweetpotato With Different Drought Tolerance
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摘要 为从蛋白水平揭示不同耐旱性甘薯柴根和块根的差异,明确甘薯根系分化和耐旱性生理机制,以耐旱性强的济薯21(JS21)和耐旱性弱的济紫薯1(JZS1)为试验材料,在旱棚内人工控水模拟田间干旱,采用i TRAQ技术开展不同耐旱性甘薯柴根和块根的全蛋白组差异蛋白分析。结果表明,共鉴定到甘薯根系差异蛋白2 003个,其中可信蛋白1 716个、动态表达蛋白819个。GO分析表明,干旱胁迫条件下,与柴根相比,JS21和JZS1块根差异蛋白分别集中于细胞质内碳水化合物合成、能量代谢等生物过程;与JZS1块根相比,JS21块根差异蛋白分子功能主要涉及过氧化物酶活性、氧化还原酶活性等。KEGG分析表明,木质素代谢在甘薯根系分化和响应干旱胁迫的过程中起到重要的调控作用。从淀粉和蔗糖代谢、苯丙烷代谢途径富集的差异蛋白分析中探明,正常条件下,JS21主要通过ADPGase积累淀粉,JZS1不仅通过ADPGase还通过UDPGase积累淀粉;干旱胁迫条件下,JS21块根中淀粉的合成仍大于分解,而JZS1块根中蔗糖的分解大于合成。正常条件下,2个品种柴根中均含有丰富的抗氧化酶;但干旱诱导JS21块根产生过氧化物酶,导致JZS1块根中木质素合成关键酶上调表达。蔗糖合酶是干旱胁迫条件下耐旱性弱的JZS1根系中能量代谢重要蛋白。综上,干旱胁迫条件下,耐旱性强的JS21可通过柴根中已有的和块根中诱导产生的过氧化物酶协同抵御干旱胁迫,而耐旱性弱的JZS1则通过蔗糖合酶维持能量代谢,其块根木质化程度加剧。本研究为甘薯耐旱性品种生理鉴定和耐旱基因发掘提供了一定的理论依据。 In order to reveal the difference between thick root(TR)and storage root(SR)of sweet potato with difference drought tolerance from protein level,and further explicit the mechanism,two varieties including Jishu21(JS21)with strong drought tolerance and Jizishu1(JZS1)with weak drought tolerance were chosen as research material.The isobaric tags for relative and absolute quantification(i TRAQ)is used to reveal differential expression proteins between thick root(TR)and storage root(SR)by artificial water to simulate moderate drought stress in rainproof shelter.The results indicated that total 2003 differential expressed proteins were identified in eight comparable groups,among which there were 1 716 reliable protein and 819 dynamic expression protein.Compared with TR,differential proteins in SR of JS21 and JZS1 under drought stress mainly focused on biological process of carbohydrate synthesis and energy metabolism which located in cytoplasm by GO analysis.But compared with SR of JZS1,the differential proteins in SR of JS21 were enriched in molecular function of peroxidase and oxidoreductase activity.KEGG analysis showed lignin metabolism played an important regulatory role in roots differentiation and drought responses.According to differential proteins analysis in starch and sucrose pathway and phenylpropanoid biosynthesis pathway,we found JS21 accumulated starch by ADPGase,but JZS1 accumulated starch by ADPGase and UDPGase under normal condition.Under drought stress,starch synthesis was greater than decomposition in SR of JS21,but the result was opposite for sucrose synthesis in SR of JZS.Diverse antioxidant enzymes existed in TR of two varieties under normal condition,which peroxidase in JS21 storage root was induced by drought and resulted key enzymes of lignin biosynthesis were up-regulated expression by drought.Sucrose synthase was considered as a vital metabolism enzyme which supplied energy for JZS1 under drought.In conclusion,under drought condition,peroxidase which had existed in TR and induced in SR could synergistically resist drought stress in JS21 with strong drought tolerance,but JZS1 with weak drought tolerance only maintained energy metabolism by sucrose synthase and lignification were aggravated.The study can provide specific clues for physiological identification of drought tolerance sweetpotato and exploration of related genes.
作者 汪宝卿 张海燕 解备涛 段文学 张立明 WANG Baoqing;ZHANG Haiyan;XIE Beitao;DUAN Wenxue;ZHANG Liming(Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100;Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100)
出处 《核农学报》 CAS CSCD 北大核心 2019年第11期2133-2146,共14页 Journal of Nuclear Agricultural Sciences
基金 国家现代农业产业技术体系建设专项(CARS-10-B08) 山东省薯类产业创新团队项目(SDAIT-16-09) 山东省农业科学院青年科研基金(2016YQN04) 山东省农业科学院农业科技创新工程(CXGC2016B04)
关键词 甘薯 干旱 柴根 块根 蛋白组学 sweetpotato drought thick root storage root proteome
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