白藜芦醇通过保护线粒体和减少氧化应激改善失血性休克大鼠肠损伤

Resveratrol improves intestinal injury in hemorrhagic shock rats by protection of mitochondria and reduction of oxidative stress

目的:探讨白藜芦醇是否能够减轻失血性休克大鼠肠损伤及其作用机制。方法:24只无特定病原体级Sprague-Dawley大鼠随机分为正常对照组(n=8)、白藜芦醇治疗组(SR组,n=8)和溶剂组(SS组,n=8),记录平均动脉压。失血性休克2 h后分别给予15 mg/kg的白藜芦醇或0.3 m L等体积的溶剂(70%乙醇)并回输自体血。治疗2 h后采集小肠标本行HE染色后,再行肠黏膜的病理学观察和评分,免疫组织化学法检测超氧化物歧化酶2(superoxide dismutase2,SOD2)表达,Western印迹测定SOD2和细胞色素C(cytochrome C,Cyt C)蛋白含量。取部分肠组织匀浆检查ATP、谷胱甘肽过氧化物酶(glutathione peroxidase,GXH-px)、过氧化氢酶(catalase,CAT)、总SOD活性。结果:自体血回输2 h后,SR组平均动脉血压高于SS组,两组间差异有统计学意义(P<0.01);SR组较SS组病理损伤减轻,且病理学评分明显下降(P<0.05);SR组小肠组织匀浆GXH-px,CAT和SOD活性以及ATP水平均高于SS组(均P<0.01);SR组SOD2蛋白水平高于SS组,胞质Cyt C水平明显低于SS组,组间比较差异有统计学意义(均P<0.01)。结论:白藜芦醇减轻了失血性休克大鼠的肠损伤,其机制可能与减少氧化应激和保护线粒体有关。

Objective: To explore whether resveratrol can reduce intestinal damage in hemorrhagic shock rats and the underlying mechanism. Methods: A total of 24 Sprague-Dawley rats of speciif c pathogen free (SPF) were randomly divided into a control group(n=8), a resveratrol group (SR group,n=8) and a vehicle group (SS group,n=8). hT e mean arterial pressure was recorded. Two hours atf er hemorrhagic shock, 15 mg/kg resveratrol or 0.3 mL equal volume of vehicle and autologous blood were given, respectively. The intestinalspecimens were collected for hematoxylin-eosin (HE) staining and calculated the pathological score. The superoxide dismutase 2 (SOD2) and cytochrome C (Cyt C) protein expression was examined by immunohistochemistry and/or Western blot. ATP level, activities of glutathione peroxidase (GXH-px), catalase (CAT) and SOD were also detected. Results: Two hours atfer autologous blood transfusion, the mean arterial pressure in the SR group was signiifcantly higher than that in the SS group (P<0.01). Compared with the SS group, the pathological injury was signiifcantly alleviated and pathological scores were dramatically reduced in the SR group (P<0.05). hTe activities of GXH-px, CAT, SOD and the ATP levels in the SR group were signiifcantly higher than those in the SS group (allP<0.01). Compared with the SS group, the SOD2 expression was significantly higher while the Cyt C expression was dramatically lower in the SR group (both P<0.01). Conclusion: Resveratrol could alleviate the intestinal injury in hemorrhagic shock rats, which might be associated with its effects on reduction of oxidative stress and protection of mitochondria.