促排卵联合PureceptionTM密度梯度法优选精子在AIH中的应用

Combination of PureceptionTM gradient centrifugation techniques for sperm separation and ovarian stimulation in AIH

目的:探讨促排卵联合PureceptionTM密度梯度法优选精子在AIH中的应用。方法:采用促排卵治疗后,用PureceptionTM密度梯度离心法处理精液,并将优选后的精子应用于AIH治疗周期。结果:精液处理后,精子密度由处理前(47.13±22.97)×109/L提高到(69.32±12.65)×109/L,活率由42.27±12.33%提高到83.20±10.21%,畸形精子率由62.42±23.12%降至38.28±17.24%,差异有显著性。59对夫妇共进行了113个AIH治疗周期,自然周期(33例)AIH妊娠率9.10%,促排卵周期(80例)AIH妊娠率15.00%,差异有显著性。结论:PureceptionTM密度梯度法是一种较好的精子优选方法,联合促排卵治疗可以获得获得较满意的AIH临床妊娠率。

Objective To evaluate the efficacy of the combination of PureceptionTM gradient centrifugation techniques for sperm separation and ovarian stimulation in AIH. Methods The semen was prepared by Purecep-tionTM gradient centrifugation after the ovarian stimulation as a treatment, and the selected sperm suspension was used for AIH. Results After preparation, the density of sperm was increased from (47.13±22.97)×109/L to (69.32± 12.65)×109/L, the motility of sperm was raised from (42.27±12.33%) to (83.20±10.21%) as well, while the abnor-mal sperm was reduced from (62.42±23.12%) to (38.28±17.24%), the differences were statistical significant (P<0.01). The study includes 59 infertile couples who received 113 AIH cycles. The clinical pregnant rate per cycle was 9.10%in nature cycles (33 cycles), and 15.00%in ovarian stimulation cycles (80 cycles). Conclusion Pure-ceptionTM gradient centrifugation technique combined with ovarian stimulation in AIH, the clinical pregnant rate is satisfactory.