心肌特异性Hole转基因小鼠模型的构建与鉴定

Establishment and identification of cardiomyocyte-specific Hole transgenic mouse model

目的:建立在小鼠心肌细胞中特异性过表达Hole转基因小鼠。方法:构建心肌特异性过表达Hole的转基因载体,显微注射导入小鼠受精卵,通过胚胎移植,获得转基因首建者小鼠。利用PCR检测Hole基因整合情况,并通过实时定量PCR检测Hole基因过表达效率。结果:PCR检测发现有6只小鼠在其基因组上整合有Hole载体基因。实时定量PCR结果显示,在心脏组织中Hole基因的转录本表达明显升高。结论:成功建立了在小鼠心肌细胞中特异性过表达Hole转基因小鼠,为研究Hole基因在心脏发育与相关疾病中的功能及机制提供了动物模型。

Objective To obtain transgenic mice that specifically over-expresses in cardiomyocytes. Meth-ods A construct that directed the over-expression of Hole to cardiomyocytes was made and 6 transgenic mice were obtained through fertilized egg microinjection followed by embryo transplantation. The integration of Hole gene was detected by PCR while the heart-specific overexpression of Hole was confirmed by real time PCR. Re-sults 6 mice carrying the Hole transgenic was identified by PCR. The results showed that Hole was over-ex-pressed in the cardiac tissue. Conclusion cardiomyocyte-specific Hole transgenic mice are generated successful-ly. The transgenic mice could be a useful model for the study in heart development and associated diseases.