TGFβ1 T29C多态性与NSCLC易感性及放射敏感性的相关性研究

Association of Polymorphism of T29C in TGFβ1 Gene with Susceptibility and Radiosensitivity of Non-Small Cell Lung Cancer in Chinese PopμLation

目的:研究湖南地区汉族人群TGFβ1 T29C多态性与非小细胞肺癌易感性及放射敏感性的关系。方法:应用聚合酶链反应技术对122例湖南籍汉族人(60例非小细胞肺癌患者及62例健康人)转化生长因子TGFβ1 T29C SNP位点进行扩增,以ABI测序直接进行PCR产物测序。统计NSCLC组及健康对照组各基因分型情况及分析基因型与放疗疗效之间相关性。结果:(1)NSCLC组和对照组基因型分布均符合HardyWeinberg遗传平衡。NSCLC组和对照组的T与C等位基因频率分别为51.7%、58.1%和48.3%、41.9%。NSCLC组的TT、TC、CC基因型频率分别为28.3%、46.7%及25.0%。对照组的TT、TC、CC基因型频率分别为33.9%、48.4%及17.7%,但两组之间比较无统计学意义。(2)NSCLC患者中TC基因型对放疗的有效率低,仅为28.6%,其次为CC基因型,为50.0%,而TT基因型的患者对放疗的有效率较高,为64.7%,三者之间的差异有统计学意义。结论:TGFβ1 T29C多态性可能与NSCLC发生无关,但NSCLC患者TGFβ1 T29C多态性可能与放射敏感性有关。

Objective To evaluate the relationship between polymorphism of T29C in TGFβ1 gene and susceptibility and radiosensitivity of non small cell lung cancer( NSCLC) in ethnic Han Chinese in Hunan province. Methods Polymerase chain reaction technology for 122 cases( 60 cases of non-small cell lung cancer and 62 cases of healthy people) of transforming growth factor TGFβ1 T29C in Hunan Province was amplified,PCR products were sequenced directly with ABI instrument. Statistics NSCLC group and the healthy control group genotyping analysis of the situation and the correlation between genotype and radiotherapy. Results 1. The tested genetic polymorphisms frequency distribution in control group and NSCLC group were in Hardy-Weinberg equilibrium law. T and C allele frequencies in NSCLC group and the control group were 51. 7%,58. 1% and 48. 3%,41. 9% respectively. TT,TC,CC genotype frequencies in NSCLC group were 28. 3%,46. 7% and 25. 0%. TT,TC,CC genotype frequency in control group was 33. 9%,48. 4% and 17. 7%,but the difference between the two groups is not significant. 2. Effective rate of radiotherapy in NSCLC patients with TC genotype was only 28. 6%,CC genotype in NSCLC patients was 50. 0%,and a higher efficiency of 64. 7% in the TT genotype in patients with radiotherapy,and the difference was significant( χ2 = 6. 125 P < 0. 05). Conclusion There is no significant relationship between the T29C SNP in TGFβ1 and susceptibility in NSCLC. The TGFβ1 T29C SNP is closely associated with radiosensitivity of NSCLC in the ethnic Han Chinese in Hunan province.