Ack1在胃癌中的表达及其临床意义

Express and Significance of Ack1 Gene in Gastric Adenocarcinoma

目的:通过检测Ack1基因在胃腺癌组织和癌旁、正常胃组织中的表达差异分析其与患者临床病例特征的相关性。方法:收集手术切除并经病理证实的胃腺癌组织石蜡标本60例作为胃癌组,癌旁组织15例及正常胃组织15例分别作为癌旁组、正常组,用免疫组织化学SP法检测胃腺癌组织与癌旁及正常组织中Ack1蛋白的表达情况,并对Ack1蛋白的表达差异及其与患者的临床病理特征用SPSS17.0软件进行统计学分析。结果:Ack1蛋白在胃癌组织中表达阳性率明显高于正常胃组织及癌旁组织。三者Ack1蛋白表达差异具有统计学意义。两两比较后认为正常胃组织与胃癌组织、胃癌组织与癌旁组织Ack1蛋白表达差异具有统计学意义,而正常胃组织与胃癌癌旁组织Ack1蛋白表达差异不具有统计学意义。再者Ack1蛋白的表达与胃癌患者的分化程度及TNM分期有关,与病人性别、年龄、肿瘤发生部位及是否淋巴结转移无关。结论:Ack1可作为一种新型胃癌标记物,在胃癌的发生及发展过程中起到重要作用,为胃癌的个体化治疗提供了实验依据。

Objective To study the gene Ack1 in gastric adenocarcinoma and its expression differences with the patient of the clinical and pathological features of relevance. Methods Collect surgical resection and confirmed by pathology gastric adenocarcinoma tissue of paraffin wax 60 cases of cancer,15 cases of the normal tissue and 15 cases of the adjacent normal gastric cancer tissue,using immunohistochemical method to detect the Ack1 coding protein expression,with SPSS17. 0 statistical software to Ack1 protein expression differences and their patients and the clinical and pathological features of the statistical analysis. Results The three Ack1 protein expression differences are statistically significant. After comparing two two think is normal stomach tissue and gastric cancer tissue,stomach tissue and cancer tissue adjacent to Ack1 protein expression are statistically significant differences,but the normal stomach tissue and cancer of the stomach cancer tissue adjacent to Ack1 protein expression differences were not significant. Ack1 expression and gastric cancer patients about the differentiation degree and TNM staging,the low differentiated the lack of the higher rate of expression; Ack1 protein expression and patient age,sex,tumor location and whether lymph node metastasis have nothing to do. Conclusion Ack1 protein expression in the tissue in gastric adenocarcinoma is obviously down-regulated or missing,and in normal tissue in the stomach common expression. Ack1 protein expression and cancer differentiation degree of relevant,differentiation degree the low loss rate is high its expression.