肥大细胞在实验性末端回肠炎发病中的变化及其意义

The Changes and Significance of Mastocyte in the Progress of Experimental Terminal Ileitis

目的:探讨肥大细胞在实验性末端回肠炎发病过程中的变化及其意义。方法:选用雄性SD大鼠,随机分为对照组、缝线组、造模组,每组20只,分别予以仅麻醉不手术、回肠末端缝手术线、回肠末端-盲肠侧侧吻合术的处理。术后2、8周分批予以处死大鼠,取末端回肠组织,分别观察其病理学改变,计数肥大细胞及其脱颗粒程度、增殖凋亡情况并分析。结果:1.SD大鼠术后2、8周肥大细胞的数量有明显的减少(F2周=63667.0,P2周=0.000;F8周=65363.0,P8周=0.000);,且造模组与缝线组相比细胞数亦减少(t2周造模=8781.082,P2周造模<0.001;t8周造模=106.670,P8周造模<0.001)。2.造模、缝线组中肥大细胞呈圆形、梭形、不规则形等多种形态改变,细胞间质可见多处点状颗粒样改变。3.SD大鼠术后2、8周肥大细胞脱颗粒程度逐渐加重(χ22周=18.207,P2周=0.000;χ28周=24.348,P8周=0.000),造模组与缝线组之间程度也有加重(P<0.05)。4.在术后2、8周缝线组、造模组增殖率逐渐下降,且两者变化的情况相似(P>0.05)。缝线组和造模组在第2周时,肥大细胞少量凋亡,第8周时,缝线组和造模组肥大细胞均可见大量细胞凋亡,而且凋亡细胞造模组明显多于缝线组。5.凋亡DNA梯度条带的变化:缝线组与造模组2、8周均出现特征性的"梯形"(200bp、400bp)条带,且造模组200bp条带较缝线组明显。结论:在实验性末端回肠炎的发病过程中,肥大细胞的数量明显减少、脱颗粒程度明显加重。这些变化可能与发病过程中肥大细胞的凋亡增多有关。

Objective To investigate the changes and significance of mastocyte in the progress of experimental terminal ileitis. Methods Male SD rats were chosen and divied into three groups randomly and equally,20 rats are included in each group,the rats in control group simply have anesthesia. The terminal ileum of suture group sewed up by suture lines. The rest rats from model group were given side-to-side anastomisis of terminal ileum and caecum,Part of rats were killed 2 and 8 weeks later,and took the tissue from terminal ileum to observe the changes of pathological,count the number of both mastocytes and their degranulation degree,to analyze the prolixferation rate and number of apoptotic cells. Results 1. In the 2nd and 8th postoperative week,mastocytes decreased obviously,( F2W= 63667. 0,P2W= 0. 000; F8W= 65363. 0,P8W= 0. 000); Model group displayed a decline of cells conmpared with suture group( t2W= 8781. 082,P2W< 0. 001; t8W= 106. 670,P8W< 0. 001). 2. In model group and suture group,mastocytes turned out to be of round,spindle and some other irregular shape. And multiple pointlike granular changes could be viewed in cellular gaps. 3. In the 2nd and 8th postoperative week,the degranulation degree went up( χ22W= 18. 207,P2W= 0. 000; χ28W= 24. 348,P8W= 0. 000),model group and with suture group revealed a larger extent of degranultion( P < 0. 05). 4. In the 2nd and 8th postoperative week,the proliferation rate was went down gradually both in model group and suture group,and the situation was much alike( P < 0. 05). In the 2nd week,model group and suture group had a small amount of Apoptosis,in the 8nd week,the two group both had the largest number of apoptotic,and model group was obviously compared with suture group. 5. Change of gradient stripes of apoptotic DNA: In the 2nd and 8th postoperative week,suture group and model group showed typical laddershaped stripes( 200 bp and 400 bp stripes),and model group even showed clearer 200 bp stripes. Conclusion In the progress of CTI,the number of mastocytes has been decreased evidently,and the degranulation degree of mastocytes has been more serious,that might be relevant to the increase of their apoptosis.