卡维地洛对扩张型心肌病大鼠心肌Cx43表达的影响

Effect of Cavedilol on the Expression of Myocardial Cx43 in Rats with Dilated Cardiomyopathy

目的:观察卡维地洛对阿霉素诱导的扩张型心肌病(dilated cardiomyopathy,DCM)大鼠心室肌缝隙连接蛋白43(connexin 43,Cx43)表达的影响。方法:应用多柔比星腹腔注射建立DCM大鼠模型;模型组大鼠按照不同的给药方法随机分为卡维地洛组和模型对照组,卡维地洛组应用卡维地洛加双蒸馏水灌胃,模型对照组和正常对照组用定量的双蒸馏水灌胃,三组均1次/d,灌胃4周。12周末超声测量各组大鼠心脏左心室射血分数(LVEF),逆转录聚合酶链反应法检测Cx43 mRNA,采用免疫组化方法观察Cx43分布。结果:与模型对照组比较,卡维地洛组大鼠心肌Cx43mRNA、Cx43平均灰度值、LVEF及均明显增高,卡维地洛组较模型对照组心肌Cx43的分布紊乱有改善,差异均有统计学意义。结论:卡维地洛能改善DCM大鼠心肌Cx43表达和分布,并能改善其心功能。

Objective To observe the effect of Cavedilol on connexin 43 in dilated cardiomyopathy ( DCM) rats induced by adriamycin .Methods Intraperitoneal injection was used to prepare adriamycin induced dilated cardiomyopathy model in Wistar rats .The rats in the model group according to different methods of administration were randomly divided into Cavedilol group and the model control group .Their LVEF were investigated with echo-cardiogram.Immunohistochemical and image analytic techniques were used to observe myocardial Cx 43 expression and distribution in different group .The Cx43 mRNA level of cells was tested by reverse transcriptase polymerase chain reaction (RT-PCR) in different group.Results Compared with the model control group ,The Cx43 level of protein, the Cx43 mRNA level of cells and LVEF increased significantly in Cavedilol group ,the distribution of myo-cardial Cx43 could improve the disorder in Cavedilol group ,Comparing the differences among the 3 groups were sta-tistically significant .Conclusion Cavedilol can improve myocardial Cx 43 expression and distribution of DCM rats, and can improve cardiac function .