DFMG对内皮细胞COX-2表达和黏附功能的影响

The Effect of 7-Difluoromethoxy-5,4'-Dimethoxygenistein on COX-2 Expression and cell adhesion in HUVE-12 Cells

目的 :观察7-二氟甲氧基-5,4'-二甲氧基金雀异黄素(DFMG)对损伤的人脐静脉内皮细胞(HUVE-12)中环氧化酶2(cyclooxygenase-2,C0X-2)蛋白表达的影响及内皮细胞的保护作用。方法 :TNF-α诱导建立人脐静脉内皮细胞(HUVE-12)损伤模型,不同浓度的DFMG进行干预;流式细胞术检测细胞凋亡、Western Blot检测COX-2蛋白表达、ELISA检测E-选择素、单核趋化蛋白-1(Monocyte chemoattractant protein-1,MCP-1)的释放,蛋白定量分析法测定内皮细胞与单核细胞的黏附率。结果 :DFMG呈浓度依赖性阻断损伤的内皮细胞活性的下降、下调COX-2蛋白的表达、降低内皮细胞与单核细胞的黏附率。结论 :DFMG可能通过下调COX-2蛋白表达水平,从而减少单核细胞/内皮细胞黏附作用,减少炎症渗出,抑制炎症反应,保护血管内皮。

Objective To observe the effect of 7-difluoromethoxy-5, 4’- dimethoxygenistein(DFMG) on the expression of COX-2 and endothelium protection in the impaired human umbilical vein endothelial(HUVE-12) cells. Methods The model of injured HUVE-12 is established by Tumor necrosis factor(TNF-α)which was dealt with different concentrations of DFMG. The apoptosis was detected by flow cytometry using Annexin V/PI staining.The expression levels of cyclooxygenase-2(COX-2) were detected by western blotting.The content of the fragments of E-selectin,monocyte chemoattractant protein-1(MCP-1) was detected by the method of ELISA, the monocyte/endothelial cell adhesion was detected by protein quantitative analysis method.Results DFMG can interdict the decline of injured HUVE-12 activation, down-regulate the expression of COX-2 and antagonize the adhesion between endothelial cells and monocytes in a concentration dependent manner. Conclusion DFMG may antagonize TNF-α-induced inflammation in HUVE-12 cells by down-regulating the expression of COX-2 which can reduce inflammatory exudation, inhibit inflammation and protect vascular endothelium from resistant atherosclerosis.