不同时间阶段慢性间断性缺氧对大鼠舌下神经放电活性的影响

Different phases of chronic intermittent hypoxia influence rats hypoglossal nerve discharge activity

目的 :观察不同时间阶段慢性间断性缺氧及复氧对大鼠舌下神经电活性的影响。方法 :将30只雄性SD大鼠随机分成五组:间断缺氧4周组(IH4组)、间断缺氧8周组(IH8组)、间断缺氧12周组(IH12组)、间断缺氧-复氧组(RH组)、对照组(UC组)。IH4组、IH8组、IH12组于实验期间,每日间断缺氧8h,氧浓度波动于5%~21%之间;RH组:间断性缺氧处理8周后,在正常氧环境下继续饲养4周;对照组:正常氧环境下饲养,其余条件同CIH组。实验开始第12周后,记录各组大鼠舌下神经放电活动。结果 :IH4、IH8、IH12组大鼠舌下神经放电活性均较UC组增高,差异有统计学意义。IH12组大鼠舌下神经放电活性较IH4组及IH8组显著减弱,但仍明显高于对照组,差异有统计学意义。RH组大鼠舌下神经放电活性较IH4、IH8、IH12组均明显下降,差异有统计学意义。与对照组比较,无统计学意义。结论 :SD大鼠经长期CIH刺激后,舌下神经放电活动的频率及振幅均呈现先升高后降低的双相变化过程。间断缺氧后再复氧,可使舌下神经电活性在失代偿前恢复至对照组水平。

Objective To observe the effect of chronic intermittent hypoxia and reoxygenation on hypoglossal nerve activity in rat. Methods 30 male SD rats were randomly divided into five groups: intermittent hypoxia 4 weeks group(IH4 group), intermittent hypoxia 8 weeks group(IH8 group), intermittent hypoxia 12 weeks group(IH12 group), reoxygenation group(RH group),unhandled control group(UC group). After the start of the experiment, in twelfth weeks, hypoglossal motor output was measured. Results Comparison with control rats, hypoglossal nerve activity was increased in IH4, IH8, IH12 rats.Comparison with IH4,IH8 rats, IH12 rat hypoglossal nerve activity was significantly decreased, but still higher than control group. hypoglossal nerve burst frequency and amplitude in group RH than IH4, IH8, IH12 groups were significantly decreased. Compared with the control group, no statistical significance. Conclusion Long-term stimulation of CIH evoke sustained augmentation of hypoglossal nerve activity and then decreased. In timely reoxygenation restored the post-hypoxia hypoglossal nerve burst frequency and amplitude decline in CIH rats.