薯蓣皂苷元干预对野百合碱诱导肺动脉高压大鼠的影响

The Effects of Diosgenin on Monocrotaline Induced Pulmonary Arterial Hypertension in Rats

目的 :探讨薯蓣皂苷元(Dio)干预对野百合碱(MCT)诱导肺动脉高压(PAH)大鼠影响。方法 :36只大鼠随机分为正常对照组、MCT模型组、肺动脉高压薯蓣皂苷元干预组,检测平均肺动脉压力(m PAP)、右心指数(RV/LV+S)、形态学指标、IL-6及凋亡指数,以观察Dio干预对MCT诱导PAH大鼠的影响。结果 :薯蓣皂苷元干预组m PAP、右心指数、肺小动脉管壁厚度、肺小动脉胶原纤维含量、肺组织匀浆中IL-6水平明显低于MCT模型组,而凋亡指数明显高于MCT模型组。结论 :Dio可能通过抑制肺血管重构、抑制炎症因子和促进肺血管平滑肌细胞凋亡等途径,防治MCT诱导的大鼠PAH。

Objective Discuss the Effects of diosgenin intervention on monocrotaline inducing pulmonary artery hypertension on rats. Methods Divide the 36 rats into normal control group, MCT model group and pulmonary artery hypertension diosgenin intervention group. Detect the average pulmonary artery pressure(m PAP), right- cardiac index(RV/LV+S), morphology index, IL-6 and apoptosis index to observe the effect of Dio intervention on MCT inducing PAH on rats. Results The m PAP, right ventricle hypertrophy index, pulmonary arteriole wall thickness, collagenous fiber content in pulmonary arteriole and IL-6level in pulmonary tissue homogenate of the diosgenin intervention group are significantly lower than those of the MCT model group, while the apoptosis index is significantly higher than that of the MCT model group. Conclusion Dio may protect rats against PAH induced by MCT, through inhibiting pulmonary vascular remodeling and inflammatory factor, and promoting pulmonary vascular smooth muscle cell apoptosis.