PHLDA3在肝细胞癌中的表达及临床意义

The clinical significance and the expression of PHLDA3 in hepatocellular carcinoma

目的 :探讨PHLDA3在肝细胞癌中的表达及其对肝细胞癌生物学行为的影响。方法 :通过实时荧光定量PCR和免疫组化检测肝细胞癌组织中PHLDA3 m RNA和蛋白的表达情况,分析PHLDA3的表达与肝细胞癌临床病理特征的关系。结果 :肝细胞癌组织中PHLDA3 m RNA和蛋白的表达均明显低于邻近非瘤肝组织,PHLDA3的表达水平与肝细胞癌患者的AFP、结节数目、血管侵犯、TNM分期和BCLC分期紧密相关。结论 :PHLDA3在肝细胞癌中低表达,与肝细胞癌的不良临床病理特征密切相关,可能是肝细胞癌侵袭转移的抑制因素。

Objective To investigate the expression of PHLDA3 in hepatocellular carcinoma and the impacts on biological behavior of hepatocellular carcinoma. Methods The expression levels of PHLDA3 m RNA and protein in hepatocellular carcinoma were detected via real-time fluorescent quantitative PCR and Immunohistochemical methods. The relationship between expression levels of PHLDA3 and Clinicopathologic features of hepatocellular carcinoma were analyzed. Results The expression of PHLDA3 m RNA and protein in hepatocellular carcinoma tissues were both significantly lower than in the adjacent nontumor liver tissue. The expression level of PHLDA3 were closely related with the AFP, nodule number, vascular invasion, TNM staging and BCLC staging in the patients with hepatocellular carcinoma. Conclusion The expression of PHLDA3 in hepatocellular carcinoma was low, and were closely related with adverse clinical pathological features in hepatocellular carcinoma patients, PHLDA3 may be the inhibiting factor for the invasion and metastasis of hepatocellular carcinoma.