骨髓间充质干细胞移植预防放射性肺损伤及相关机制

Research on the Prevention of Radiation Lung Injury by Bone Marrow Mesenchymal Stem Cell Transplantation and the Mechanism

目的 :探讨骨髓间充质干细胞移植对放射性肺损伤的防治作用及相关机制。方法 :80只雌性SD大鼠随机分为A(单纯干细胞组),B(单纯照射组),C(干细胞治疗组),D(空白对照组)。B、C组大鼠建立放射性肺损伤模型;A、C组大鼠经尾静脉注射1mL BMSCs。第8、12周取各组大鼠外周血及肺组织,观察并分析干细胞移植对放射性肺损伤及相关分子表达的影响。结果 :TSPY基因在C组大鼠肺组织中的表达显著升高。C组的肺泡炎性反应较B组轻,各组均未观察到明显的肺纤维化,但免疫组化可见C组促纤维化因子TNF-α、TGF-β1较B组低表达;C组SP-B、Arg-1、SOD的表达显著高于B组,而iNOS的表达显著低于B组。结论 :(1)BMSCs尾静脉移植可有效减轻大鼠的放射性肺损伤。(2)TNF-α、TGF-β1、SOD、iNOS、Arg-1分子表达水平的改变在其过程中起到重要作用。

Objective To investigate the preventive effect of bone marrow mesenchymal stem cells transplantation on radiation-induced lung injury and try to figure out the mechanism. Methods 80 female SD rats were randomly divided into four groups: group A were only injected with 1 ml BMSCs through tail vein; group B only accepted radiation; group C accept radiation and injection both; group D were black control. Effection of stem cell transplantation on radiation-induced lung injury and the associated molecular changes were observed after 8 and 12 weeks after treatment. Results Group C showed significantly stronger expression of TSPY gene in lung tissue than other groups and showed less of alveolar inflammation than group B. However, pulmonary fibrosis was observed in none group. Nevertheless, compare to group B, the expression of fibrosis factor(TNF-a, TGF-beta1) and iNOS were depressed, and the expression of SP-B, Arg-1 and SOD were upregulated. Conclusions(1) BMSCs transplantation through tail vein reduced radiation-induced lung injury in rats effectively.(2) Molecular changes of TNF-alpha, TGF-beta 1, SOD, iNOS and Arg-1 should be an important part of the mechanism.