胃癌患者血清sHLA-E和s HLA-G的表达水平及临床意义

Serum levels and clinical significance of sHLA-E and sHLA-G in gastric cancer patients

目的 :可溶性HLA分子可促进肿瘤免疫逃逸,从而影响恶性疾病的临床病程。本研究旨在探索胃癌患者分泌sHLA-E和sHLA-G的情况,以及其分泌水平可能受哪些因素影响,探讨两指标作为肿瘤相关标志物的潜在价值。方法 :入组70岁以下的胃癌患者56例,健康者48例,使用ELISA法检测他们sHLA-E、sHLA-G的水平,比较两组人sHLA-E、sHLA-G水平及阳性率的差异。收集胃癌患者性别、年龄和分期资料,分析sHLA-E、sHLA-G水平与这些因素之间的关系。使用IFN-γ、IL-1β、IL-2以及IL-6刺激人胃腺癌细胞AGS,ELISA法检测其上清HLA-E水平。结果 :不同年龄、性别胃癌患者血清HLA-E、HLA-G水平无统计学差异,不同分期患者sHLA-E水平有统计学差异,sHLA-G水平无统计学差异。sHLA-E水平在Ⅲ期患者明显高于Ⅰ~Ⅱ期和Ⅳ期患者。胃癌患者血清HLA-E、HLA-G水平明显高于健康人,两指标阳性率也明显高于健康人。IFN-γ可诱导人胃腺癌细胞AGS分泌HLA-E,而IL-1β、IL-2以及IL-6均无明显诱导作用。结论 :血清HLA-E水平及阳性率在胃癌患者明显高于健康人。sHLA-E水平与胃癌分期相关,其分泌可被IFN-γ诱导,可能是一种潜在的临床标志物。

Objective Soluble HLA molecules can promote tumor immune escape and thus affect the clinical course of malignant diseases.This study aims to investigate sHLA-E and sHLA-G secretion by gastric cancer and the factors that may affect the secretion level for addressing their potential value as tumor related markers.Methods This study enrolled 56 gastric cancer patients under 70 and 48 healthy donors.ELISA was used to detect the serum level of HLA-E and HLA-G.Both levels and positive rates of serum HLA-E and HLA-G were compared between two groups.We collected the data of gender,age and stage,and analyzed the relationship between them and sHLA-E,sHLA-G levels.We detected the soluble HLA-E and HLA-G secreted by human gastric adenocarcinoma cell AGS that was co-incubated with IFN-γ,IL-1β,IL-2 and IL-6.Results There was no statistical difference in sHLA-E,sHLA-G levels between gastric cancer patients and healthy people.Serum HLA-E levels were statistically variant in patients with different stages,whereas HLA-G no variance.Serum HLA-E levels in stageⅢpatients were significantly higher than that in stageⅠ-ⅡandⅣ.Both serum levels and positive rates of HLA-E and HLA-G were significantly higher in gastric cancer patients than healthy people.IFN-γinduced the secretion of HLA-E by human gastric adenocarcinoma cell AGS,whereas IL-1β、IL-2 and IL-6 do not.Conclusion Serum HLA-E level was significantly increased in gastric cancer patients and associated with disease stage,which may be a potential clinical biomarker.