miRNA-21过表达对乳鼠星形胶质细胞凋亡及Wnt/β-catenin信号通路相关蛋白表达的影响

Effects of miRNA-21 overexpression on apoptosis and expressions of Wnt/β-catenin signaling pathway related proteins in astrocytes from neonate rats

目的:探讨miRNA-21(miR-21)过表达对乳鼠星形胶质细胞(AS)凋亡及Wnt/β-catenin信号通路相关蛋白表达的影响。方法:AS分为3组,空白对照组、H_2O_2组(400μmol/L的H_2O_2处理AS 24 h)和miR-21+H_2O_2组(miR-21 mimics转染AS 24 h后用400μmol/L的H_2O_2处理细胞24 h)。采用qRT-PCR检测3组细胞miR-21的表达,流式细胞术检测细胞凋亡,Western blot检测β-catenin、c-myc和Bax蛋白的表达。结果:与空白对照组比较,H_2O_2组miR-21表达降低,细胞凋亡率升高,β-catenin和c-myc表达降低,Bax表达升高(P <0. 05);与H_2O_2组比较,miR-21+H_2O_2组miR-21表达升高,细胞凋亡率降低,β-catenin和c-myc蛋白表达升高,Bax蛋白表达降低(P <0. 05)。结论:过表达miR-21可降低AS凋亡,其机制可能与Wnt/β-catenin信号通路的激活有关。

Aim: To investigate the effect of miRNA-21( miR-21) gene overexpression on the apoptosis of astrocytes and the expressions of Wnt /β-catenin signaling pathway-related proteins. Methods: Astrocytes were divided into 3 groups, namely,blank control group,H2O2 group and miR-21 + H2O2 group. qRT-PCR was used to detect the expression of miR- 21 mRNA, flow cytometry was used to detect cell apoptosis, and Western blot was used to detect the expressions of β-catenin, c-myc and Bax protein. Results: Compared with blank control group, the expression of miR-21 in the H2O2 group decreased, the apoptosis rate increased,the expressions of β-catenin and c-myc decreased,and the expression of Bax increased( P < 0. 05);compared with the H2O2 group,the miR-21 expression in the miR-21 + H2O2 group increased,the apoptosis rate decreased,the expressions of β-catenin and c-myc increased,and the expression of Bax decreased( P < 0. 05) . Conclusion: Overexpression of miR-21 could reduce the apoptosis of astrocytes,which may be related to the activation of Wnt /β-catenin signaling pathway.