黄芪多糖对HCT116细胞侵袭和迁移能力的影响

Effects of astragalus polysaccharides on invasion and migration of colorectal cancer HCT116 cells

目的:研究黄芪多糖对人结直肠癌HCT116细胞侵袭和迁移能力的影响,并初步探讨其作用机制。方法:体外培养人结直肠癌HCT116细胞,分别以0. 0、0. 1、0. 2,0. 4、0. 6、0. 8和1. 0 g/L的黄芪多糖干预HCT116细胞24 h,采用MTT法检测HCT116细胞的存活情况。采用0. 0、0. 1和0. 6 g/L黄芪多糖作用HCT116细胞24 h后,Transwell和划痕实验检测侵袭和迁移能力,采用Western blot检测细胞中基质金属蛋白酶(MMP)-2、MMP-9蛋白表达水平。结果:黄芪多糖能够剂量依赖性抑制HCT116细胞增殖。0. 6 g/L黄芪多糖处理HCT116细胞24 h后,细胞侵袭和迁移能力,MMP-2、MMP-9蛋白的表达水平均显著下降(P <0. 05)。结论:黄芪多糖能够抑制HCT116细胞侵袭和迁移,其作用机制可能与下调MMP-2、MMP-9的表达有关。

Aim: To study the effects of astragalus polysaccharides( APS) on invasion and migration of human colorectal cancer HCT116 cells, and to explore its mechanism. Methods: HCT116 cells were cultured in vitro, and treated with different concentrations ( 0. 0,0 . 1,0 . 2,0 . 4,0 . 6,0 . 8 and 1. 0 g /L) of APS. The growth ability of HCT116 cells was detected by MTT assay after 24 h treatment, and the concentration of APS in the subsequent experiments was screened. HCT116 cells were treated with 0. 0,0 . 1 and 0. 6 g /L APS for 24 h, then Transwell and scratch test was used to detect the invasion and migration of HCT116 cells. The expression levels of matrix metalloproteinase-2 ( MMP-2) and matrix metalloproteinase-9 ( MMP-9) in HCT116 cells were detected by Western blot. Results: APS could inhibit the proliferation of HCT116 cells in a dose-dependent manner. The results of Transwell and scratch test showed that the invasiveness and migration ability of HCT116 cells treated with 0. 6 g /L APS was significantly lower than that of the control group( P < 0. 05);APS could significantly inhibit the expression levels of MMP-2 and MMP-9 protein( P < 0. 05) . Conclusion: APS can inhibit the invasion and migration of HCT116 cells,which may be related to the down-regulation of MMP-2 and MMP-9 expression.