Notch信号在慢性牙周炎实验大鼠模型的表达研究

Expressionof notch signal in ratsmodel of chronic periodontitis

目的:建立SD大鼠慢性牙周炎模型,采用实时定量聚合酶链反应(RT-PCR)、流式细胞术对慢性牙周炎模型外周血单个核细胞(PBMC)中Notch信号通路,以及其相关受体的表达水平进行研究,希望能找到Notch相关分子在慢性牙周炎发生发展中的潜在意义,能够为寻求新的治疗慢性牙周炎的方法提供理论基础。方法:27只6～8周龄雄性SD大鼠,随机分成对照组和模型组。通过牙龈卟啉单胞菌,建立慢性牙周炎大鼠模型,观察记录大鼠牙龈指数、牙的松动度、牙周袋深度,采用荧光定量聚合酶链反应和流式细胞术从不同水平检测模型组大鼠PBMC中Notch信号通路相关受体(Notch1～Notch4)的表达量,并与对照组比较。结果:成功建立了SD大鼠慢性牙周炎模型。模型组Notch1、Notch3、Notch4的mRNA和细胞表达百分比明显低于对照组(P<0.01),而Notch2的mRNA和细胞表达百分比的表达明显高于对照组(P<0.05)。结论:Notch信号通路对慢性牙周炎免疫平衡失衡起重要的调控作用,为深入研究慢性牙周炎的发病机制提供了思路。

Objective: To establish a chronic periodontitis model of SD rats and detect the expression of Notch in peripheral blood mononuclear cells(PBMCs) of chronic periodontitis model was detected by real-time quantitative polymerase chain reaction(RT-PCR),intracellular staining and flow cytometry. It is hoped that we can find out the potential significance of Notch related molecules in the development of chronic periodontitis and provide a theoretical basis for seeking new methods of treating chronic periodontitis.Methods:27 male SD rats aged 6-8 weeks were randomly divided into control group and model group. By means of Porphyromonasgingivalis, a chronic periodontitis rat model was established. The gingival index, tooth mobility and periodontal pocket depth were recorded. The expression levels of Notch signaling related receptors(Notch1 to Notch4) in PBMC of model mice were detected by fluorescence quantitative polymerase chain reaction, flow cytometry and intracellular staining at different levels and compared with the control group. Results:The model of chronic periodontitis in SD rats was successfully established. The expression ofmRNA of Notch1, Notch3,Notch4 and percentage of cell expressionin the model group were significantly lower than that in the control group(P<0.01), but Notch2 mRNA and percentage of cell expressionwere significantly higher(P<0.05).Conclusion:This indicated that Notch and its ligands may play important roles in imbalance of immune balance for chronic periodontitis and provide ideas for in-depth study of the pathogenesis of chronic periodontitis.