大肠埃希菌感染的糖尿病溃疡大鼠模型建立及评价

Establishment and evaluation of diabetic foot Ulcer rat model infected by Escherichia coli

目的:建立大肠埃希菌感染的糖尿病溃疡大鼠模型,为糖尿病溃疡实验研究提供合适的动物模型。方法:将32只SD雄性大鼠随机分为4组,普通溃疡组(N组)、糖尿病溃疡组(DU组)、模型组(M组)、贝复济组(B组),造模后取局部皮肤组织行HE染色、采用酶联免疫吸附(ELISA)检测造模后大鼠血清中高敏C反应蛋白(High sensitive c-reactive protein,HCRP)、成纤维细胞生长因子(basic fibroblast growth factor, bFGF)含量以及创面愈合后HCRP、bFGF、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)、糖基化终末产物(advanced glycation end products,AGES)、白细胞介素-1β(Interleukin-1β,IL-1β)、白细胞介素-6(Interleukin-6,IL-6)含量水平,并记录创面愈合时间,对该模型的稳定性、实用性进行评估。结果:造模后N组HCRP含量低于其他3组(F=40.140,P<0.001);各组bFGF含量无统计学差异(F=1.846,P>0.05)。创面愈合后各组大鼠血清中HCRP含量具有统计学差异(F=6.340,P=0.003),组间比较B组低于M组(P=0.001);各组bFGF含量比较无差异(F=2.331,P>0.05),组间比较B组高于N组(P=0.018);各组AGEs含量比较具有统计学差异(F=18.662,P=0.000),组间比较B组低于DU组、M组(P=0.006、0.000);各组TNF-α、IL-1β、IL-6含量比较有差异(F=174.820、62.80、16.008),组间比较B组TNF-α、IL-1β、IL-6水平均低于M组(P=0.000、0.000、0.17);M组与DU组相比,TNF-α、IL-1β水平较高(P=0.000、0.004)。创面愈合时间比较,M组愈合时间较N组、DU组、B组明显延迟,差异具有统计学意义(F=93.443,P<0.05)。结论:该模型较符合感染性糖尿病溃疡局部环境,为糖尿病感染的研究提供了一种可靠的工具。

Objective: It is expected to establish a diabetic ulcer rat model infected with Escherichia coli to provide an appropriate model for the experimental study of diabetic foot ulcer. Method: 32 SD male rats were randomly divided into three groups including the common ulcer group( N), the diabetic ulcer group(DU) and the model group(M) and Befuji group(B). The stability and practicability of the model were evaluated by HE performed on the local skin tissue of the wounds and ELISA detection detecting the content of High sensitive c-reactive protein(HCRP), basic fibroblast growth factor(bFGF)in rat serum after modeling and HCRP, bFGF, tumor necrosis factor-alpha(TNF-α), advanced glycation end products(AGEs), Interleukin-1β(IL-1β) and Interleukin-6(IL-6) levels after wound healing. Results: After modeling, the HCRP content in N group was lower than that in the other three groups( F=40.140,P<0.001). There was no significant difference in bFGF content between the groups(F=1.846,P>0.05).After wound healing, the HCRP content in serum of rats in each group showed statistical difference( F=6.340, P=0.003).The level of HCRP in group B was lower than that in group M( P=0.001). There was no difference in bFGF content between the groups(F=2.331, P>0.05).The level of bFGF in group B was higher than that in group N(P=0.018). The contents of AGEs in each group were statistically different(F=18.662,P=0.000).The contents of AGEs in group B were lower than those in group DU and group M(P=0.006, 0.000). The contents of TNF-α, IL-1β and IL-6 in each group were different(F=174.820, 62.80, 16.008,P all<0.001). The levels of TNF-α, IL-1βand IL-6 in group B were lower than those in group M(P=0.000,0.000,0.17). Compared with DU group, the levels of TNF-α,IL-1βin M group were higher(P=0.000,0.004). Wound healing time in group M was significantly delayed than that in group N, groupDU and group B(F=93.443, P<0.05).Conclusion: This model is more in line with the local environmental characteristics of infected diabetic ulcer and provides a reliable tool for the study of diabetic infections.