摘要
目的:探讨不同浓度的黄芪甲苷对乌头碱诱导的斑马鱼心脏损伤的保护作用。方法:挑选受精后发育正常的48 h斑马鱼胚胎,用30 mg/L的乌头碱溶液药浴,同时给予10、25、40μg/m L的黄芪甲苷溶液,观察斑马鱼的心率、心脏形态以及静脉窦-动脉球(SV-BA)距离的变化,观察黄芪甲苷对其心脏损伤的保护作用。结果:30 mg/L的乌头碱溶液处理24 h可致斑马鱼心率加快、心包水肿、SV-BA间距延长。25、40μg/m L黄芪甲苷溶液能降低斑马鱼的心率,减轻斑马鱼心包水肿的程度。结论:黄芪甲苷对乌头碱诱导的斑马鱼心脏损伤具有一定的保护作用,且呈剂量依赖性。
Objective:To investigate the protective effect of astragalosideⅣat different concentrations on aconitine-induced zebrafish heart injury.Methods:Zebrafish of 48 hours post fertilization were treated with 30 mg/L aconitine solution and 10,25,and 40μg/m L astragalosideⅣsolution for 24 h.We observed the heart rate,heart shape,and SV-BA of zebrafish.Results:The 30 mg/L aconitine solution can cause zebrafish heart injury such as heart rate increase,ventricular arrest,pericardial edema.The study found that 25 and 40μg/m L astragalosideⅣsolution can reduce the heart rate of zebrafish and relieve the extent of pericardium edema.Conclusion:The AstragalosideⅣshows a certain protective effect on aconitine-induced cardiac damage in zebrafish,which is dose-dependent.
作者
石亚楠
王佑华
李强
姚磊
符德玉
SHI Ya-nan;WANG You-hua;LI Qiang;YAO Lei;FU De-yu(Yueyang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200080,China;Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Translational Medical Center for Development and Disease Institute of Pediatric,Shanghai Key Laboratory of Birth Defect Children's Hospital of Fudan University,Shanghai 201102,China)
出处
《中医药导报》
2019年第7期26-28,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
黄芪甲苷
斑马鱼
乌头碱
心脏损伤
心率
AstragalosideⅣ
zebrafish
aconitine
heart damage
heart rate
