摘要
目的:建立桂枝饮片指纹图谱方法,分析不同产地桂枝饮片质量。方法:色谱柱为费罗门Luna C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液,柱温:30℃,流速:1.0 m L·min-1,检测波长:254 nm。采用相似度评价、聚类分析方法,对15批样品进行比较与分析。结果:15批桂枝样品中指认了6个共有峰,相似度在0.90以上,并分为2类。结论:该方法简便、可靠,可以用于不同产地桂枝饮片质量控制与评价。
Objection:To establish the method of HPLC fingerprinting and to analyze the quality of Guizhi(Cinnamomi Ramulus)in different areas.Methods:The column was a Phenomenex Luna(2)C18 column(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.1%phosphoric acid solution.The column temperature was30℃,and the flow rate was 1.0 m L/min.The detection wavelength was 254 nm.Ten peaks were extracted as the common peaks of 15 batches of samples.The similarity evaluation,cluster analysis and principal component analysis were used to compare and analyze 15 batches of samples.Results:A total of six peaks were extracted as the common peaks of 15 batches of samples.The similarity was 0.90 or more.And the 15 batches of samples were divided into two categories.Conclusion:The method is simple and reliable,and can be used for quality control and evaluation of Guizhi(Cinnamomi Ramulus)in different areas.
作者
孙福仁
李军山
常云凤
李雪丽
冀艳花
李振江
SUN Fu-ren;LI Jun-shan;CHANG Yun-feng;LI Xue-li;JI Yan-hua;LI Zhen-jiang(Shineway Pharmaceutical Group Ltd.,Shijiazhuang Hebei 051430,China;TCM Formula Granule Engineering Technology Research Center of Hebei,Shijiazhuang Hebei 050200,China)
出处
《中医药导报》
2019年第7期55-57,64,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家中医药管理局中医药科学技术研究专项课题(国中医药科2013X07)
关键词
桂枝
指纹图谱
聚类分析
Guizhi(Cinnamomi Ramulus)
HPLC fingerprint
clustering analysis