丁苯酞对脑梗死大鼠迁徙蛋白2表达的影响

Effect of butylphthalide on the expression of Slit2 protein after cerebral infarction in rats

目的探讨丁苯酞对脑梗死大鼠迁徙蛋白2(secreted leucine-rich repeat-containing protein 2,Slit2)表达的影响及其在脑梗死中的可能作用。方法 SD大鼠90只随机分为模型组、药物组和对照组各30只。模型组、药物组采用线栓法制备大鼠局灶性脑梗死模型,对照组颈总动脉不插入线栓;模型制备成功后,药物组给予丁苯酞120 mg/kg+花生油稀释至2 mL灌胃1次,模型组、对照组给予等量花生油灌胃。灌胃后1、3、7d,评定3组神经行为学评分,并分别处死10只大鼠,采用ELISA法检测3组脑组织Slit2表达水平。结果灌胃后1、3、7d,模型组神经行为学评分[(2.43±0.21)、(1.32±0.15)、(0.61±0.15)分]高于药物组[(1.50±0.24)、(0.41±0.12)、(0.03±0.01)分]和对照组[0、0、0分](P<0.05);灌胃后1、3、7d,药物组、模型组神经行为学评分逐渐降低;灌胃后1、3 d,药物组神经行为学评分高于对照组(P<0.05),灌胃后7 d与对照组比较差异无统计学意义(P>0.05);灌胃后1、3、7 d,药物组Slit2水平[(0.35±0.02)、(0.51±0.04)、(0.83±0.05)μg/L],模型组Slit2水平[(0.22±0.01)、(0.34±0.02)、(0.50±0.01)μg/L]高于对照组[(0.15±0.02)、(0.15±0.02)、(0.15±0.02)μg/L],且药物组Slit2水平高于模型组(P<0.05)。结论丁苯酞可能通过促进Slit2表达来减轻脑梗死大鼠脑损伤程度,促进脑功能恢复。

Objective To explore the possible effect of butyphthalide on cerebral infarction by analyzing the expression of secreted leucine-rich repeat-containing protein 2(Slit2) in rats with cerebral infarction.Methods Ninety SD rats were randomly divided into control group,model group and drug group,with 30 rats in each group.Model group and drug group were established models of focal cerebral infarction by thread embolization.In control group,the common carotid artery was not inserted with thread embolization.Drug group received gavage with butylphthalide(120 mg/kg) diluted in peanut oil to 2 mL,while model group and control group received gavage with equivalent peanut oil.Neurological behavior scores were assessed by day 1,3 and 7 after gavage in three groups,and meanwhile 10 rats in each group were sacrificed.The expression of Slit2 was detected by ELISA.Results The neurological behavior scores were significantly higher in model group(2.43±0.21,1.32±0.15,0.61±0.15) than those in drug group(1.50±0.24,0.41 ±0.12,0.03±0.01) and control group(0,0,0) by day 1,3 and 7 after gavage(P<0.05),and decreased gradually with the administration time in drug group and model group(P<0.05).The neurological behavior scores were significantly higher in drug group than those in control group by day 1 and 3 after gavage(P<0.05),and showed no significant difference by day 7 after gavage between two groups(P>0.05).The levels of Slit2 by day 1,3 and 7 after gavage were significantly higher in drug group((0.35±0.02),(0.51±0.04),(0.83±0.05) μg/L) and model group((0.22±0.01),(0.34±0.02),(0.50±0.01) μg/L) than those in control group((0.15±0.02),(0.15±0.02),(0.15±0.02) μg/L)(P<0.05),and higher in drug group than those in model group(P<0.05).Conclusion Butylphthalide may reduce the degree of brain injury to promote the recovery of brain function by increasing the expression of Slit2.