非神经性烟碱型乙酰胆碱受体在直肠癌组织中的表达及意义

Expression of non-neuronal nicotinic acetylcholine receptor in colorectal cancer tissue and its significance

目的探讨非神经性烟碱型乙酰胆碱受体(α7nicotinic acetylcholine receptor,α7nAChR)在直肠癌组织中的表达及临床意义。方法直肠癌患者72例,取其手术切除直肠癌组织及癌旁组织标本,采用免疫组织化学SP法检测α7nAChR阳性表达率,Spearman法分析α7nAChR阳性表达与临床病理特征的相关性。结果肿瘤组织α7nAChR阳性表达率(69.4%)高于癌旁组织(16.7%)(P<0.05);α7nAChR阳性表达率在肿瘤Dukes分期A+B期者(62.0%)低于C+D期者(86.4%),高分化+中分化者(60.4%)低于低分化+未分化者(87.5%),肿瘤直径<5cm者(63.0%)低于≥5cm者(80.8%)(P<0.05);Spearman相关分析结果显示,Dukes分期C+D期、低分化+未分化、肿瘤直径≥5cm与α7nAChR阳性表达呈明显正相关(r=0.553,P=0.021;r=0.489,P=0.032;r=0.533,P=0.029)。结论α7nAChR在直肠癌组织中呈高表达,且其阳性表达与原发病灶低分化+未分化、肿瘤直径≥5cm、Ducks分期C+D期明显相关。

Objective To investigate the expression of non-neuronal α7 nicotinic acetylcholine receptor(α7nAChR) in colorectal cancer tissue and its clinical significance.Methods The expression rate of α7nAChR in resected rectal cancer and adjacent tissues from 72 patients with rectal cancer were detected by SP immunohistochemical method,and were analyzed the relationship between the positive expression of α7nAChR and clinical pathological characteristics by Spearman method.Results The positive rate of α7nAChR was significantly higher in tumor tissue(69.4%)than that in adjacent tissue(16.7%)(P<0.05),in tumor Dukes stage A+B(62.0%)than that in Dukes stage C+D(86.4%),in those with high and moderate differentiation(60.4%)than that in those with low differentiation and undifferentiation(87.5%),and in those with <5cm in diameter(63.0%)than that in those with ≥5cm in diameter(80.8%)(P<0.05).Spearman correlation analysis showed that Dukes stage C+D,low differentiation and undifferentiation,and diameter≥5cm were obviously correlated with positive expression of α7nAChR(r=0.553,P=0.021;r=0.489,P=0.032;r=0.533,P =0.029).Conclusion α7nAChR is highly expressed in rectal cancer tissue,and the positive expression is correlated with low differentiation and undifferentiation of primary tumor,diameter≥5cm and Ducks stage C+D.