夏桑菊颗粒不同干燥方式对指标成分含量的影响

Effect of Different Drying Methods of Xiasangju Granule on Content of Index Component

目的:优选夏桑菊颗粒浸膏的干燥方式。方法:夏桑菊颗粒药材经提取、浓缩后制得浸膏,将浸膏均分成3份,每份分别采用真空干燥、喷雾干燥、微波干燥等干燥方式,将干燥后的浸膏粉采用高效液相色谱法进行绿原酸、迷迭香酸以及蒙花苷的含量测定。色谱条件:色谱柱为Waters Symmetry Shield RP18(5μm,4. 6 mm×250. 0 mm)柱,以乙腈(A)-(体积分数) 1. 0%醋酸溶液(B)为流动相梯度洗脱,流速为0. 9 m L·min^(-1),柱温为35℃,检测波长为320 nm。结果:绿原酸、迷迭香酸以及蒙花苷的线性范围分别为0. 050 4～1. 260 0μg、0. 031 2～0. 780 0μg、0. 051 2～1. 280 0μg,平均加样回收率分别为98. 3%(RSD=1. 16%)、98. 6%(RSD=1. 72%)、98. 0%(RSD=1. 01%);真空干燥、喷雾干燥和微波干燥所得样品中,绿原酸的含量分别为0. 259 mg·g^(-1)、0. 307 mg·g^(-1)、0. 284 mg·g^(-1),迷迭香酸的含量分别为0. 361mg·g^(-1)、0. 383 mg·g^(-1)、0. 396 mg·g^(-1),蒙花苷的含量分别为0. 166 mg·g^(-1)、0. 230 mg·g^(-1)、0. 216 mg·g^(-1)。结论:该方法可用于评价夏桑菊颗粒不同干燥方式的优劣,3种干燥方式对指标成分的影响为:真空干燥>微波干燥>喷雾干燥。

Objective: To optimize the drying method of Xiasangju granules extract. Methods: Extracted and concentrated extracts of Xiasangju medicinal materials were prepared. The extracts were divided into 3 parts. Each of them was dried by vacuum drying,spray drying,microwave drying,etc.,and the dried extract powder was applied to the high performance liquid phase. Chromatography was performed for the determination of chlorogenic acid,rosmarinic acid,and linicalin. Chromatographic conditions: The column was a Waters Symmetry Shield RP18( 5 μm,4. 6 mm × 250. 0 mm) column and eluted with acetonitrile( A)-( volume fraction) 1. 0% acetic acid solution( B) as the mobile phase gradient. The flow rate was 0. 9 m L·min-1,The column temperature is35 ℃,and the detection wavelength is 320 nm. Results: The linear ranges of chlorogenic acid,rosmarinic acid and linicalin were0. 050 4-1. 260 0 μg,0. 031 2-0. 780 0 μg,and 0. 051 2-1. 280 0 μg,respectively. The average recovery was 98. 3%( RSD =1. 16%),98. 6%( RSD = 1. 72%) and 98. 0%( RSD = 1. 01%);the contents of chlorogenic acid in the samples dried by vacuum drying,spray drying and microwave drying were 0. 259 mg·g-1,0. 307 mg·g-1and 0. 284 mg·g-1respectively. The content of rosmarinic acid was 0. 361 mg · g-1,0. 383 mg·g-1,and 0. 396 mg·g-1respectively. The content of linicalin was 0. 166mg·g-1,0. 230 mg·g-1,and 0. 216 mg·g-1respectively. Conclusion: The established method can be used to evaluate the advantages and disadvantages of different drying methods for Xia Sangju granules. The effect of the three drying methods on the index components is: vacuum drying > microwave drying > spray drying.