腹泻高热大鼠模型的实验研究

An experimental study of the rat model of diarrhea and severe fever

目的:探索运用中医"下法"结合腹腔注射大肠杆菌内毒素(lipopolysaccharides,LPS)复制腹泻高热大鼠模型的可行性。方法:以SD雄性成年大鼠为研究对象,分为灌胃0、1、3、5、7d组,采用序贯给药法,分别灌胃给予大黄芒硝水煎液0、1、3、5、7d,于末次灌胃后经腹腔注射给予LPS。观察并记录造模大鼠粪便硬度与体温变化情况,考察灌胃大黄芒硝所致的腹泻及天数对经LPS诱导大鼠体温的影响。结果:大鼠灌胃大黄芒硝水煎液后,整体状态变差,粪便硬度下降,粪便含水量增加。仅灌胃大黄芒硝对大鼠体温无明显影响。大鼠腹腔注射LPS后体温均明显升高。其中灌胃大黄芒硝5d的大鼠注射LPS后体温升高所需时间短,且峰值最高。结论:大鼠经灌胃大黄芒硝水煎液后再腹腔注射LPS可成功建立高热模型,大黄芒硝水煎液的灌胃天数对大鼠的发热潜伏期、发热持续时间和体温峰值均有影响。

Objective: To investigate the feasibility of the traditional Chinese medicine( TCM) purgation method combined with intraperitoneal injection of lipopolysaccharide( LPS),an endotoxin of Escherichia coli,in establishing a rat model of diarrhea and severe fever. Methods: Adult male Sprague-Dawley rats were selected and divided into 0-,1-,3-,5-,and 7-day gavage groups. The drugs were administered sequentially;water decoction of Rheum officinale and mirabilite was given by gavage for 0,1,3,5,and 7 days,and intraperitoneal injection of LPS was performed after the last time of administration by gavage. The changes in stool consistency and body temperature were observed and recorded,and the influence of diarrhea due to Rheum officinale and mirabilite given by gavage and its duration on the body temperature of rats treated by LPS was analyzed. Results: After being treated with the water decoction of Rheum officinale and mirabilite by gavage,the rats had poor conditions,with a reduction in stool consistency and an increase in fecal water content. Rheum officinale and mirabilite given by gavage alone had no significant influence on body temperature. There was a significant increase in body temperature in rats after intraperitoneal injection of LPS,and the rats treated with Rheum officinale and mirabilite by gavage for 5days had the shortest time to the increase in body temperature after LPS injection and the highest peak value of body temperature. Conclusion: Water decoction of Rheum officinale and mirabilite by gavage followed by intraperitoneal injection of LPS can successfully establish a rat model of diarrhea and severe fever,and the number of days of treatment with water decoction of Rheum officinale and mirabilite by gavage may affect the latency,duration,and peak body temperature of fever.