侧柏叶乌发活性部位的实验研究

An experimental study of the active part of Cacumen biotae with a hair-blackening effect

目的:筛选侧柏叶乌发的活性部位。方法:采用蘑菇酪氨酸酶多巴速率氧化法测定侧柏叶不同极性部位及其不同浓度对酪氨酸酶活性的影响,评价侧柏叶不同极性部位及不同浓度的乌发效果。结果:4批侧柏叶中石油醚部位0. 5g/ml的剂量有上调蘑菇酪氨酸酶活性的作用。结论:侧柏叶中具有乌发作用的可能是石油醚部位,且与其浓度有关,高浓度有上调蘑菇酪氨酸酶活性的作用。

Objective:To investigate the active part of Cacumen biotae with a hair-blackening effect.Methods:The method of oxidation of L-dopa by mushroom tyrosinase was used to observe the influence of different polar parts of Cacumen biotae at different concentrations on the activity of tyrosinase,and the hair-blackening effect of different polar parts of Cacumen biotae at different concentrations was evaluated.Results:Among the 4 batches of Cacumen biotae,the petroleum ether extract with a dose of 0.5g/ml upregulated the activity of mushroom tyrosinase.Conclusion:The petroleum ether extract of Cacumen biotae may exert a hair-blackening effect in a dose-dependent manner,and a high concentration of this extract can upregulate the activity of mushroom tyrosinase.