解郁降压颗粒结合音乐疗法对围绝经期高血压病大鼠性激素水平的影响

Effect of Jieyu Jiangya granules combined with music therapy on the levels of sex hormones in a rat model of perimenopausal hypertension

目的:观察解郁降压颗粒结合音乐疗法对围绝经期高血压病模型大鼠性激素的调节作用,初步探讨解郁降压颗粒结合音乐疗法治疗围绝经期高血压病的可能机制。方法:选用动情周期规律的SPF级雌性自发性高血压(SHR)大鼠,将其随机分成SHR组、假手术组、围绝经期高血压病模型组(简称模型组)、解郁降压颗粒加音乐疗法组(简称治疗组);另设WKY大鼠作为正常血压对照组。通过摘除双侧卵巢方法建立围绝经期高血压病模型,待造模成功后,予以相应干预。治疗结束后测量血清雌二醇(E2)、促卵泡生成素(FSH)、促黄体生成素(LH)的含量,同时测量大鼠动脉血管的平均光密度值(OD值)。结果:与SHR组比较,治疗组血压下降,差异有统计学意义(P <0. 05);与模型组比较,治疗组E2上升,OD值上升,FSH、LH含量下降,差异均有统计学意义(P <0. 05)。结论:解郁降压颗粒结合音乐疗法对围绝经期高血压病模型大鼠有降压作用,且能提高SHR模型大鼠血清E2的含量,降低FSH、LH含量,改善SHR模型大鼠的内分泌环境;OD值上升,表示雌激素α受体(ERα)水平提高;而对内分泌环境的改善和ERα水平的提高可能是其降压机制之一。

Objective:To investigate the regulatory effect of Jieyu Jiangya granules combined with music therapy on sex hormones in a rat model of perimenopausal hypertension,as well as the possible mechanism of Jieyu Jiangya granules combined with music therapy in the treatment of perimenopausal hypertension.Methods:Specific pathogen-free female spontaneous hypertension rats(SHRs)with a regular estrous cycle were selected and randomly divided into SHR group,sham-operation group,perimenopausal hypertension model group(model group),and Jieyu Jiangya granules+music therapy group(treatment group).WKY rats with normal blood pressure were selected as control group.A rat model of perimenopausal hypertension was established by resection of both ovaries,and corresponding intervention was given after the model was established successfully.After treatment,the serum levels of estradiol(E2),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)were measured,and the mean optical density(OD)value of arteries was also measured.Results:Compared with the SHR group,the treatment group had a significant reduction in blood pressure(P<0.05),and compared with the model group,the treatment group had significant increases in E2 and OD value and significant reductions in FSH and LH(P<0.05).Conclusion:Jieyu Jiangya granules combined with music therapy exert an antihypertensive effect on rats with perimenopausal hypertension and can increase the serum level of E2,reduce the serum levels of FSH and LH,and improve endocrine environment in SHR model rats.An increase in OD value suggests an increase in estrogen receptorα(ERα),and improvement of endocrine environment and an increase in ERαlevel may be one of the antihypertensive mechanisms of this treatment.