基于iTRAQ技术的冠心病血瘀证蛋白质谱研究

Study of Protein Mass Spectrometry of Coronary Heart Disease with Blood Stasis Syndrome Based on iTRAQ Technology

目的探索冠心病血瘀证差异蛋白质谱,从蛋白水平阐释冠心病血瘀证的生物学机制。方法对冠心病血瘀证与非血瘀证两组,应用同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,i TRAQ)技术分离、鉴定两组的差异蛋白质。结果鉴定到的蛋白质数量780个。两组共有27个蛋白点表达相差1.5倍以上,其中11个上调,16个下调。上调的差异蛋白中主要功能涉及免疫反应、细胞间粘附作用主要指血小板参与的凝血反应、脂质代谢。下调的差异蛋白中主要功能涉及血小板细胞变形、细胞与细胞间关系迁移、细胞损伤表达。结论冠心病血瘀证与免疫反应、血小板参与凝血反应的亢进,以及影响血小板形态变化、血小板间、血管内皮细胞与血小板的粘附迁移有密切的关系。

Objective To explore the differential protien mass spectrum of coronary heart disease(CHD) with blood stasis syndrome, and its biological mechanism. Methods The diffrential proteins in blood stasis syndrome and non-blood stasis syndrome groups were isolated and identified by isobaric tags for relative and absolute quantitation(i TRAQ) technology. Results780 proteins were identified. In the two groups, the expression difference of 27 protein spots was 1.5 times higher, and 11 of them were up-regulated and 16 of them were down-regulated. The up-regulated diffrential proteins were related with immune response,blood coagulation and lipid metabolism. The function of down-regulated proteins was related to platelet cytomorphosis, metastasis between cells, Conclusion The CHD with blood stasis syndrome has close relation with immune response, platelet participate in the coagulation reaction of hyperthyroidism, as well as with the influence of platelet morphology change, platelets, vascular endothelial cells and platelets adhesion and migration.