复方血栓通对脑缺血再灌注模型大鼠脑MDA和SOD的影响

Effect of Compound Xueshuantong on MDA and SOD in Rats with Cerebral Ischemia Reperfusion

目的观察评价复方血栓通对脑缺血再灌注模型大鼠脑活性和丙二醛(MDA)、超氧化物歧化酶(SOD)的影响。方法实验分为6组,即假手术组、模型组、复方血栓通高剂量组、复方血栓通中剂量组、复方血栓通低剂量组、阳性药华佗再造丸组。对各组大鼠采用可逆性大脑中动脉线栓法制备局灶性脑缺血再灌注模型,进行神经功能评分,并测定脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)含量。结果假手术组与模型组比较,大鼠脑缺血再灌注损伤后脑组织MDA含量显著地降低(t=9.2476,P=0.0000),脑组织SOD显著地增高(t=21.5083,PP=0.0000);各用药组与模型组比较,阳性药华佗再造丸和复方血栓通高、中、低剂量组均能显著地降低MDA活性(分别t=3.8193、5.7638、4.9027、3.9261,P P=0.0000);阳性药华佗再造丸和复方血栓通高、中剂量组均能显著地升高SOD活性(分别t=14.2716、16.0538、15.0472,PP=0.0000);复方血栓通高剂量组升高SOD活性作用强于阳性药华佗再造丸组(t=7.4863,P=0.0000),缺血后各组动物均出现神经功能损害症状,阳性药华佗再造丸和复方血栓通高、中、低剂量组神经功能缺损评分均明显的高于假手术组(分别t=3.1728、6.4251、5.0413、3.9805,PP=0.0000);复方血栓通高剂量组神经功能缺损评分显著地低于模型组(t=6.9072,P=0.0000)。结论复方血栓通具有保护大鼠脑缺血再灌注损伤的作用,可以显著地增加脑组织SOD含量的活性,并有效的降低MDA水平减轻了脑缺血再灌注损伤,更好的发挥其对机体的保护作用。

Objective To observe the effects of compound Xueshuantong on brain activity and malondialdehyde(MDA)and superoxide dismutase(SOD)in rats with cerebral ischemia-reperfusion injury.Methods The experiment was divided into 6 groups,namely sham operation group,model group,compound thrombus high dose group,compound thrombus middle dose group,compound thrombus low dose group,and positive drug Huajing rebuilding group.A reversible middle cerebral artery occlusion method was used to prepare a model of focal cerebral ischemia-reperfusion in each group.Neurological function scores were performed,and malondialdehyde(MDA)and superoxide dismutase(SOD)in brain tissue were measured.content.Results Compared with the model group,the MDA content in brain tissue of rats with cerebral ischemia-reperfusion injury was significantly lower than that of the model group.(t=9.2476,P=0.0000).The SOD of brain tissue was significantly increased(t=21.5083,P P=0.0000).Compared with the model group,the positive drug Huayu Zaizao Pill and the compound thrombus high,medium and low dose groups significantly reduced MDA activity(respectively.t=3.1728,6.4251,5.0413,3.9805,P P=0.0000).The neurological deficit score of the compound thrombus high dose group was significantly lower than that of the model group(t=6.9072,P=0.0000).Conclusion Compound Xueshuantong has the protective effect on cerebral ischemia-reperfusion injury in rats.It can effectively increase the activity of SOD in brain tissue and reduce the level of MDA.It can alleviate the cerebral ischemia-reperfusion injury and better protect its body.