摘要
就滇中一带云南松林进行了多次调查 ,共采集、鉴定标本 1 30余号 ;从中选取 1 3科 ,1 9属 ,33种真菌进行了菌种分离、培养的研究。选用并修改 4种培养基进行了外生菌根真菌分离、培养的比较 ,结果共有 1 1科 ,1 7属 ,2 5种真菌分离获得成功 ,成功率高达 75 .8% ,4种培养基的分离成功率依次为 36.4%、63.6%、33.3%和 5 7.6%。其中 ,修改PDA培养基和松针、玉米培养基较适宜于云南松外生菌根真菌的分离、纯化和培养。
Several surveys were carried out under Pinus yunnanensis Franch forest in Central Yunnan; the total collections are over 130. 33 species, belonging to 19 genera, 13 families were selected to supply a diversity of pure cultures. 4 types of media were selected for the pure culture study of ectomycorrhizal fungi. As a result, pure cultures of 25 species, 17 genera, and 11 families were obtained. The total isolation success rate reached 75.8%; the success rate of 4 media (No.1, No2, No.3, No.4) was 36.4%, 63.6%, 33.3% and 57.6% respectively. Modified PDA medium (No.2) and pine needles medium (No.4) are more suitable for the isolation, purification and maintenance of ectomycorrhizal fungi associated with P. yunnanensis.
出处
《植物研究》
CAS
CSCD
北大核心
2003年第1期66-71,共6页
Bulletin of Botanical Research
基金
国家自然科学基金 (No 30 0 70 0 0 4)
云南省自然科学基金 (No 2 0 0 0C0 0 6 8M )
关键词
纯培养
云南松
外生菌根真菌
分离培养
Pinus yunnanensis Franch
ectomycorrhizal fungi
pure culture
