无载体固定化黄花蒿细胞生产青蒿素(英文)

SELF-IMMOBILIZED AGGREAGE CULTURE OF ARTEMISIA ANNUA L. FOR IMPROVED ARTEMISININ

疟疾是一种严重危害人类健康的流行病 ,主要由疟原虫经蚊虫叮咬引起。目前 ,在临床上疟原虫对治疗疟疾的药物 (如氯奎等 )有较强的耐药性 ,并表现出明显的交叉耐药性。来自黄花蒿的青蒿素具有极其明显的抗疟活性 ,成为临床首选的药物 ,因此青蒿素的获取成为关键。本研究采用无载体固定化法培养黄花蒿生产青蒿素 ,初步研究了无载体固定化细胞的生长特性。检测发现 ,利用该方法生产的青蒿素是常规细胞培养法的 9倍 ,因此该方法有望成为青蒿素生产的首选方法。

Malaria is a kind of serious pandemic which is caused by plasmodia through mosquito's sting and bite. At present, drugs of curing malaria are: chloroquine, ethylamine pyrimidine et al, but the most serious question is anti-drug character of plasmodia on those drugs, particular to chloroquine. Furthermore, the anti-chloroquine plasmodia strain have cross-anti character in clinical. Artemisinin from Artemisia annua L. has evidently anti-malaria activity and become the best drug in clinical. At present, the key question is that how to acquire artemisinin. For this reason we utilize self-immobilized aggregate culture of Artemisia annua L. to produce Artemisinin, and primarily studied character of self-immobilized culture of Artemisia annua L. cell. Through determining, it find that this method can acquire 9-times quantity artemisinin more than normal method. Therefore this method may be possible became the best method of artemisinin produce.