培养条件对齿瓣延胡索愈伤组织生长及延胡索乙素含量的影响

EFFECTS OF CULTURE ENVIRONMENT ON CALLUS GROWTH AND DL-TETRAHYDRO-PALMATINE CONTENT IN CULTURES OF CORYDALIS REMOTA

研究了不同培养条件对齿瓣延胡索 (Corydalisremota)愈伤组织生长及延胡索乙素(THP)含量的影响。结果表明 ,2 ,4-D较NAA更有利于提高培养物鲜重 ,加速细胞增殖。培养基添加 2 .0mg/L 2 ,4-D和 1 .0mg/LBA不仅有利于干物质积累 ,而且显著提高THP水平。蔗糖不仅可提高培养物鲜重 ,而且与半乳糖组合可显著促进干物质积累和THP水平的提高。水解酪蛋白和水解乳蛋白均有利于愈伤组织生长和THP含量的提高 ,45 0mg/L的水解乳蛋白处理组THP含量最高。光照可提高培养物鲜重和THP含量。

The effects of culture environment on callus growth and content of dl-tetrahydro-palmatine (THP) in the cultures from stem tubers of Corydalis remota were discussed. The results showed that 2,4- D was better for growth of callus cells than NAA. The dry weight of the callus and level of THP were promoted when 2 mg/L 2,4-D and 1 mg/L BA were added to the medium. Sucrose increased the fresh weight of callus,and the combination of sucrose and galactose promoted the increase of callus dry weight and raise of THP level,significantly. Either casein hydrolysate (CH) or lactolbumin hydrolysate (LH) was favored to the growth of callus and raise of THP level,and there was highest level of THP in the group treated with 450 mg/L LH. Light enhanced the growth of callus and raise the level of THP.