摘要
用合成的谷氨酸十二烷基酯核糖醇对来源于Candidarugosa的脂肪酶包衣后在有机溶剂中催化月桂酸和月桂醇的酯化反应。确定了最佳反应条件 :制备包衣酶用缓冲液的最适pH为 6 8,温度 30℃ ,有机溶剂为异辛烷。酯化反应符合单底物醇抑制的Ping -PangBi-Bi机制。在最适反应条件下 ,月桂酸和月桂醇的米氏常数分别为 2 9 1mmol·L- 1和 71 4mmol·L- 1,月桂醇的抑制常数为 1 1 3mmol·L- 1,最大反应速率为 0 6 6mmol·min- 1·g- 1。
The lipase from Candida rugosa was coated with synthetic didodecanyl glutamate ribitol and used to catalyze esterification of lauric acid and lauryl alcohol in organic solvents.The optimal reaction conditions were determined,which are buffer pH for lipase coating,6 8;reaction temperature,30℃;solvent,isooctane.The kinetics of the esterification reaction obeyed the Ping-Pang Bi-Bi mechanism with an inhibition by lauryl alcohol.The kinetic parameters under the optimal conditions were determined.The Michaelis constants of lauric acid and lauryl alcohol were 29 1 and 71 4mmol·L -1 ,respectively.The maximum rate was 0 66mmol·min -1 ·g -1 .The inhibition constant of lauryl alcohol was 11 3mmol·L -1 .The results predicted by the kinetic model were in good agreement with experiment.
出处
《化学工业与工程》
CAS
2002年第6期417-421,452,共6页
Chemical Industry and Engineering
基金
国家自然科学基金资助项目 (2 9986 0 31)
