包涵体蛋白的变复性研究

Refolding of Genetic Engineering Protein Expressed as Inclusion Bodies

针对基因工程蛋白在E.coli中表达多为无活性包涵体的特点,介绍了包涵体洗涤和去折叠的方法、蛋白质体外折叠的过程和机理、体外复性技术以及复性蛋白的检测,着重讨论了最近发展起来的蛋白质复性新技术.

The genes, of interest are often expressed as inactive inclusion bodies in Escherichia coli, so the methods for resuspending and unfolding inclusion bodies, the pathway and technique of protein refolding in vitro and the determination of renatured protein was overviewed. Especially, the new technique of protein refolding recently developed was emphasized.