喹啉黄中各组分的定性及定量研究

Qualitative and Quantitative Method of Components in Quinoline Yellow

利用高效液相色谱(HPLC)对喹啉黄各组分进行分离并定量,通过液相色谱–串联质谱(LC–MS/MS)对各组分进行定性。以0.01 mol/L乙酸铵溶液和甲醇为流动相进行梯度洗脱,利用Agilent XDB–C18色谱柱对喹啉黄各组分进行分离,峰面积归一化法定量。质谱采用电喷雾负离子(ESI–)模式电离,多反应监测(MRM)模式检测,对喹啉黄各组分进行定性。结果表明,喹啉黄主要成分为喹啉黄一钠盐和喹啉黄二钠盐,分别各有两种同分异构体,各组分质量浓度在5.0~100.0μg/m L范围内与峰面积呈良好的线性关系。加标回收率为98.2%~99.1%,测定结果的相对标准偏差为0.90%~1.58%(n=6)。该方法具有良好的精密度和准确度,可用于喹啉黄中各组分的测定。

The content of each composition of quinoline yellow was determined by using high performance liquid chromatography(HPLC). The qualitative of each components in quinoline yellow was performed by using liquid chromatography–tandem mass spectrometry. The target compounds were separated on Agilent XDB C18 column with methanol–0.01 mol/L ammonium acetate solution by gradient elution mode, and then were qualifi ed by tandem mass spectrometry with multiple reaction monitoring(MRM) mode under negative electrospray ionization mode. The results showed that the main components of quinoline yellow were mono-sulfates and di-sulfates which had two isomers, respectively. The composition content of quilonine yellow were obtained by area normalization method. The mass concentration of each constituent showed good linearity with the peak area in the range of 5.0–100.0 μg/m L. The recovery was 98.2%–99.1%, and the relative standard deviation of determination results was 0.90%–1.58%(n=6). The method has good precision and accuracy, and it can be used for the determination of quinoline yellow.