常压电喷雾离子化的机理及应用

The Principles and Applications of Electrospray-Based Ambient Ionization

作为最有前景的分析仪器之一,质谱技术已在药物、食品、环境、人类健康、国家安全及相关领域展现出广阔的应用前景。不同种类的分析物具有多种特征,这为直接离子化及质谱分析增加了难度。常压敞开式离子源是近年来新兴的一种离子源,这类离子源具有无需复杂的样品前处理、操作方便、快速、非破坏性、灵敏度及特异性好、能实现实时原位、高通量分析等特点。本文综述了基于电喷雾离子化(ESI)原理的各种离子源的电离机理、特征及应用,展望了常压敞开式离子源的发展趋势。

As one of the most promising analytical instruments,mass spectrometry( MS) technology has shown a broad application prospect in medicine,food,environment,human health,national security and other related fields. While the different types of analytes have diverse characteristics and largely add the difficulties of direct ionization and mass spectrometric analysis. Ambient mass spectrometry ( AMS ) is a kind of newly emerging technology performed under ambient conditions that allows the direct analysis of sample or sample surfaces with little or no sample pretreatment. The development and applications of ambient ionization mass spectrometry( AIMS) that realized ionization under ambient conditions without sample pretreatment have become a frontier field in mass spectrometry and deserved much attention over the last few years. Ambient ionization has high sensitivity and specificity. It also can rapidly analysis real - time in situ and achieve high-throughput analysis without destructing the sample. As a soft ionization method,ESI offers unique advantages for proteomics by allowing the direct analysis of thermolabile compounds and forming multiply charged ions. The developments and applications of mass spectrometry imaging( MSI) have become a frontier field in mass spectrometry and molecular imaging. Some recent contributions on the development of ambient ionization can be applied to mass spectrometry imaging. This review focuses on the development of ionization mechanism,characteristics and applications of various ion sources,which are based on electrospray ionization ( ESI)