固本增骨方对卵巢切除模型大鼠骨代谢和骨微结构的影响

Effects of Guben Zenggu Decoction on Bone Metabolism and Bone Microstructure in Ovariectomized Rats

目的观察固本增骨方对卵巢切除大鼠骨代谢和骨微结构的影响,探讨固本增骨方防治绝经后骨质疏松症的作用机制。方法采用卵巢切除法建立大鼠骨质疏松模型,随机分为模型对照组,戊酸雌二醇组,固本增骨方高、中、低剂量组,选取同龄大鼠作为空白对照组。用药干预12周后,腹腔麻醉,心脏采血,应用酶联免疫吸附法检测大鼠血清雌二醇(E2)、骨钙素(BGP)、Ⅰ型前胶原羧基端交联端肽(PICP)、Ⅰ型胶原吡啶交联终肽(ICTP)、抗酒石酸酸性磷酸酶-5b(TRAP-5b)水平;采用微型CT影像系统,对各组大鼠右侧股骨标本进行骨组织计量学分析。结果与空白对照组比较,模型对照组大鼠E2、骨特异性碱性磷酸酶(BALP)含量及相对骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)、连接密度(Conn.D)均降低(P<0.05),BGP、ICTP、PICP、TRAP-5b及骨小梁分离度(Tb.Sp)、结构模型指数(SMI)明显升高(P<0.05);给予药物干预后,与模型对照组相比,戊酸雌二醇组和固本增骨方各组E2、BALP及BV/TV、Tb.Th、Tb.N、Conn.D均增高(P<0.05),BGP、ICTP、PICP、TRAP-5b水平及Tb.Sp、SMI均下降(P<0.05);且固本增骨方高剂量组优于固本增骨方低剂量组(P<0.05),呈量效依赖关系。结论固本增骨方可改善卵巢切除大鼠模型骨代谢及骨微结构,从而发挥绝经后骨质疏松症的防治作用。

Objective To test the effects of Guben Zenggu Decoction on bone metabolism and bone microstructure in ovariectomized rats for the purpose of preventing and treating postmenopausal osteoporosis.Methods Osteoporosis rat models were established by ovariectomy.The model rats were randomly divided into control,estradiol valerate treatment,and Guben Zenggu Decoction treatment groups with high,medium and low dosages.After 12 weeks of treatments,10 rats from each group were randomly selected for cardiac blood sampling after abdominal anesthesia.The serum levels of estradiol(E2),osteocalcin(BGP),carboxyterminal of typeⅠprocollagen(PICP),collagen typeⅠpyridine crosslinking peptide(ICTP)and acid tartaric acid phosphatase-5 b(TRAP-5 b)were determined by ELISA.Bone histomorphometric analysis was performed on the right femoral specimen of rats using micro-CT imaging.Results The models rats had lower levels of E2,bone alkaline phosphatase(BALP)and relative bone volume fraction(BV/TV),trabecular thickness(Tb.Th),number of trabeculae(Tb.N)and connectivity density(Conn.D),and higher levels of BGP,ICTP,PICP,TRAP-5 b and degree of trabecular separation(Tb.Sp),structural model index(SMI)than their normal counterparts(P<0.05).Estradiol valerate and Guben Zenggu Decoction treatments increased the levels of E2,BALP,BV/TV,Tb.Th,Tb.N,and Conn.D significantly(P<0.05).Higher doses of Guben Zenggu Decoction resulted in higher changes(P<0.05).Conclusion Guben Zenggu Decoction can improve bone metabolism and bone micro-structure in ovariectomized rats,thus playing a preventive and therapeutic role in postmenopausal osteoporosis.