Taqman技术检测NG、CT、Uu的临床研究

THE CLINICAL APPLICATION OF TAQMAN TECHNIQUE IN DETECTION NG、CT AND Uu

目的 :评价Taqman技术检测临床标本中主要性病病原体的应用价值。方法 :应用Taqman技术、常规PCR技术及培养法检测 10 0份性病科门诊患者生殖泌尿道拭子标本 ,比较三种方法检测淋病奈瑟菌 (NG) ,沙眼衣原体 (CT) ,解脲支原体 (Uu)的灵敏度。 5 0 0份性病科门诊患者生殖泌尿道拭子标本同时经Taqman技术及常规PCR技术检测 ,结果不符者使用培养法验证 ,以比较Taqman技术及常规PCR技术的特异性。结果 :在 10 0份临床标本检测中 ,Taqman技术、常规PCR及培养法检出阳性率分别为 4 1% ,35 % ,2 1%。在 5 0 0份送检标本中 ,Taqman技术检出阳性标本 2 0 4份 ,常规PCR检出阳性标本 181份 ,其中结果不符的 4 1份标本经重复培养验证 ,37份与Taqmm技术检测结果相符。结论 :Taqman技术具有良好的敏感性和特异性 ,集PCR扩增、杂交及荧光自动化检测于一体 ,实验过程简便、快速、易于质控 ,是检测性病病原体的有效方法。

Objective:To evaluate the application value of Taqman technique in detection pathogens in sexually transmitted diseases.Methods:100 samples from uropoiesis department were detected by Taqman technique, routine PCR and culture method comparison the sensitivity of the three methods to detect NG,CT and Uu-DNA. 500 samples were detected by Taqman technique and routine PCR simultaneously, the samples of uncorrespondence were tested by culture method, comparison the specificity of Taqman technique and routine PCR.Results:The positive rates of Taqman technique, routine PCR and culture method were 4%,35% and 21% respectively. 204 positive samples were tested by Taqman technique and 181 positive samples were tested by routine PCR in 500 samples. 41 samples of uncorrpondence were tested by culture method and the results of 37 samples correspond with Taqman technique.Conclusion: Taqman technique shows higher specificity and sensitivity, it is simple and rapid, is a good method for measuring pathogens in sexually transmitted diseases.