海绵体神经损伤所致ED大鼠模型建立(英文)

Rat model of erectile dysfunction caused by cavernous nerve ablation

目的 寻找大鼠海绵体神经并建立神经损伤所致ED大鼠模型。方法 对 2 0只大鼠进行解剖 ,在外科显微镜下找到海绵体神经并经电刺激试验证实。随后将 4 2只实验大鼠随机分为假手术对照组、单侧海绵体神经损伤组及双侧海绵体神经损伤组。术后 3周用阿朴吗啡试验来评估所建动物模型。结果 盆主要神经节位于背侧前列腺后外侧叶表面 ,其最大的传出神经就是海绵体神经。诱发阴茎勃起的电刺激参数是 :电压 5V、刺激频率 2 0Hz及刺激时间 5ms。术后 3周 ,阿朴吗啡均能诱发对照组大鼠阴茎勃起 ,30分钟内平均勃起次数为 2 5 7± 1 4 0 ,实验组大鼠 ,无论单侧损伤还是双侧损伤 ,均丧失勃起功能 (0 0 0± 0 0 0 )。结论 大鼠较大的盆主要神经节及海绵体神经易于辨认 ,电刺激反应明显 ,而且大鼠价格便宜 ,易于饲养及购买是建立海绵体神经损伤性ED模型的理想动物。此外还发现 ,无论是单侧海绵体神经损伤还是双侧损伤 ,损伤后早期 。

OBJECTIVE: To identify the rat cavernous nerve and establish a rat model of erectile dysfunction (ED) caused by injury of the cavernous nerve. METHODS: Twenty rats underwent dissections. Cavernous nerves were identified with the aid of an operating microscope and confirmed by electrical stimulation. Then, 42 experimental rats were randomized into 3 groups, including sham-operated controls and unilateral and bilateral cavernous nerve ablation groups. Three weeks after surgery, rat models were evaluated with the Apomorphine test. RESULTS: The major pelvic ganglion lies on either side of the dorsolateral lobes of the prostate.It includes 2 inflows, one called hypogastric nerve and the other, the pelvic nerve. The largest outflow is termed the cavernous nerve. Stimulus parameters which could induce obvious penile erection were 5 volts, a frequency of 20 Hertz and a duration of 5 milliseconds. Three weeks after surgery, apomorphine could induce penile erection of each rat in the controls with a mean of 2.57 +/- 1.40 erections in 30 minutes, while there were no erections (0.00 +/- 0.00) in either the unilateral or bilateral groups. CONCLUSIONS: The rat large ganglion and its cavernous nerve can be easily identified and it produces an obvious response to electrical stimulation. Rats are low cost animals to purchase, easy to house and very available making an ideal animal for developing an ED model caused by cavernous nerve injury. In addition, our study showed that in the early period of cavernous nerve ablation, unilateral and bilateral treated rats lost erectile capacity.