摘要
OBJECTIVE: To evaluate whether deletion of chromosome 14q is involved in the carcinogenesis of primary glioblastoma multiforme and to identify possibly common deletion regions. METHJODS: Fourteen fluorescent dye-labeled polymorphic markers were used and polymerase chain reaction-based microsatellite analysis was employed to investigate loss of heterozygosity (LOH) on chromosome 14q in 20 primary glioblastoma multiforme (GBM). RESULTS: Ten of twenty (50%) GBM displayed LOH at one or more of the markers on chromosome 14q. Five tumors showed either LOH or non-informative on all markers tested. The most frequent LOH was observed at locus D14S65 (57.1%) on 14q32.1, and in the chromosomal region spanning from D14S63 (47.1%) to D14S74 (46.7%) on 14q23-31. None of the informative loci exhibited microsatellite instability. CONCLUSIONS: Allelic deletion on chromosome 14q plays an important role in the pathogenesis of GBM. Chromosomal regions at locus D14S65 on 14q32.1 and spanning from D14S63 to D14S74 on 14q23-31 may harbor multiple tumor suppressor genes associated with GBM.
目的 评价染色体 14q的 (杂合性 )丢失是否与原发性多形性胶质母细胞瘤的发生发展有关 ,并确定 14q上可能存在的共同杂合性丢失区域。方法 应用聚合酶链反应 (PCR)为基础的微卫星分析方法 ,采用 14个荧光标记的多态性 (微卫星 )标记 ,检测了2 0例原发性多形性胶质母细胞瘤 (GBM)染色体 14q的杂合性丢失 (LOH)状况。结果 5 0 % (10 /2 0例 )GBM在染色体 14q上至少有一个微卫星标记存在LOH ,其中 5个病例的所有被检测标记都表现为LOH或者不能提供信息。在位于 14q32 1的D14S6 5位点、14q2 3 31的D14S6 3～D14S74位点间区域检测到的LOH率最高 ,分别为 5 7 1%、4 6 7%～ 4 7 1%。在本研究中的所有位点 ,均未检测到微卫星不稳定。结论 染色体 14q上的等位基因丢失可能在GBM发病机制中起着重要作用 ,14q32 1上的D14S6 5位点、14q2 3 31上的D14S6
