心房颤动病人心房肌浆网Ca^(2 +)-ATPase及IP_3-I受体mRNA表达变化的研究(英文)

Changes of sarcoplamic reticular Ca^(2+)-ATPase and IP_3-I receptor mRNA expression in patients with atrial fibrillation

研究房颤病人肌浆网钙操纵基因 -Ca2 + ATPase(SERCA)、及IP3 I受体 (IP3R1)mRNA表达的改变。方法 38例风心病二尖瓣狭窄接受外科手术者 ,手术时取右心房及右心耳约各约 10 0mg ,通过RT -PCR(逆转录-聚合酶链反应 )技术 ,以GAPDH为内参照 ,测量SERCA及IP3R1mRNA表达水平的变化。结果 房颤者肌浆网IP3R1mRNA较窦性心律者上调 ,而SERCA却下调 ,心房不同部位无差别。结论 房颤病人IP3R1上调 ,SERCA下调 ,说明肌浆网SERCA及IP3R1与房颤的发生或维持有关。

OBJECTIVE: To investigate changes in the expression of sarcoplamic reticular Ca(2+)-ATPase (SERCA) and IP(3)-I receptors (IP(3)R(1)) mRNA in patients with atrial fibrillation. METHODS: Thirty-eight patients with mitral stenosis undergoing open heart surgery were studied. 100 mg of atrial tissue was obtained during surgery from the right appendage and the right atrium. The amount of messenger ribonucleic acid (mRNA) amount of SERCA and IP(3)R(1) was measured by reverse transcription-polymerase chain reaction (RT-PCR) and normalized to the mRNA levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). RESULTS: Levels of mRNA expression of SERCA in patients with AF, as compared with subjects in sinus rhythm, was lower and that of IP(3)R(1) was higher. The longer AF was sustained, the higher the levels of mRNA. There was no significant difference between right atrial free wall and right appendage. CONCLUSIONS: The expression changes of SERCA and IP3R mRNA may correlate with the initiation or maintenance of AF.