尿液中端粒酶活性在尿路上皮癌的诊断及监测复发中的意义(英文)

Telomerase activity in urine in diagnosis and recurrence surveillance of urothelial carcinoma

目的 探讨尿液中端粒酶活性在尿路上皮癌的诊断和监测复发中的意义。方法 采用PCR ELISA法对 5 4例尿路上皮癌尿液中端粒酶活性进行了检测 ,并观察了其中 2 3例术后尿液中端粒酶活性的变化 ,以 4 6例良性泌尿系统疾病患者作为对照组。结果 尿路上皮癌病人尿液中端粒酶活性明显增高 (P <0 0 0 1) ,肿瘤切除后恢复正常 ,在膀胱镜发现肿瘤复发前再次上升 ;随着肿瘤恶性程度的增加尿液中端粒酶活性逐渐升高 ;术前尿液中端粒酶活性与早期复发无关。结论 尿液中端粒酶活性的检测在尿路上皮癌的诊断中有参考价值 ,与肿瘤恶性程度有关 ,敏感度高于膀胱镜检查 。

OBJECTIVE: To investigate the significance of telomerase activity in urine in the diagnosis and recurrence surveillance of urothelial carcinoma. METHODS: Telomerase activity in urine of 54 cases of urothelial carcinoma (urothelial carcinoma group) was estimated by polymerase chain reaction-enzyme-linked immunosorbent assay, and monitored continuously in 23 cases after tumor removal. 46 patients with benign urological diseases were also included as the control group. RESULTS: The telomerase activity in urine of patients with urothelial carcinoma increased significantly as compared with the control group (P