24-表油菜素内酯对浒苔(Ulva prolifera)内源植物激素及相关生理影响

EFFECTS OF 24-EPIBRASSINOLIDE ON PHYTOHORMONE VARIATION AND RELATED PHYSIOLOGICAL RESPONSE OF ULVA PROLIFERA

采用植物激素的微量LC-MS检测等方法,研究了24-表油菜素内酯(EBR)对宁波浒苔(Ulva prolifera)内源植物激素含量及其叶绿素荧光参数、可溶性蛋白和糖含量、抗氧化酶活性等的影响。结果表明,浒苔体内含有吲哚乙酸、赤霉素、玉米素、反式玉米素核苷、异戊烯腺嘌呤核苷、茉莉酸等多种促生长性内源植物激素,但不含有异戊烯腺嘌呤。内源植物激素对24-表油菜素内酯的响应程度不同,大多数植物激素在处理第1天含量变化最快。低浓度24-表油菜素内酯处理时,各种内源植物激素的含量增高,均呈现先升高后降低的趋势。0.2 mg/L 24-表油菜素内酯处理组的效果最明显。在该浓度下,24-表油菜素内酯对生长相关激素含量、最大光能转化效率(Fv/Fm)和相对电子传递速率(ETR)、可溶性蛋白和可溶性糖含量、POD活性等均有较大的促进作用;在0.1 mg/L 24-表油菜素内酯处理组中,浒苔的体外抗氧化能力最强。

Trace LC-MS method was applied to determine algal phytohormone, and the effects of 24-epibrassinolide(EBR) on endogenous phytohormone content, chlorophyll fluorescence parameters, soluble protein and carbohydrate contents, and antioxidant capacity of macroalgae Ulva prolifera in Ningbo, China were investigated. Results revealed many types of phytohormones such as IAA, GA, ZR, TRZ, iPA, and JA but iP. The phytohormones showed different sensitivity to EBR, and most of them responded on the first day of treatment. Under low concentration of EBR treatment, all endogenous phytohormone contents in U. prolifera increased, showing a tendency of first increase then decrease. The 0.2 mg/L EBR group showed the best effect of promoting phytohormone, Fv/Fm(indicative of photochemical efficiency), ETR, soluble protein and soluble sugar contents, and POD of U. prolifera. However, the strongest in-vitro antioxidative capacity was detected in the 0.1 mg/L EBR group.