摘要
OBJECTIVE: To investigate the cross-reactivity between glutamic acid decarboxylase (GAD)-I-A(g7) and I-A(d) tetramer in diabetes-prone non-obese diabetic (NOD) mice (I-A(g7)) and diabetes-free Balb/c mice (I-A(d)). METHODS: Two GAD peptide I-A(g7) and I-A(d) tetramers were generated and compared for phenotype and function of sorted GAD peptide I-A(g7) and I-A(d) tetramer-positive (tet+) T cells. RESULTS: The cross-reactivity is shown in either tetramer positive percentage or tetramer staining intensity. The NOD and Balb/c derived-tet+ T cells were able to be cross-stained by GAD peptide I-A(g7) and I-A(d) tetramers, and responded to both irradiated NOD and Balb/c splenotyes under stimulation by synthetic and recombinant GAD peptides. CONCLUSION: Although I-A(g7) and I-A(d) are closely related in biochemical and biological aspects, their most notable difference is the presence or absence of a negatively charged residue at position beta57 that links to insulin-dependent diabetes mellitus.
目的 研究糖尿病易感性NOD小鼠 (I Ag7)和非糖尿病性Balb/c(I Ad)中谷氨酸去羰基酶 (GAD) I Ag7andI Ad四聚体的交叉反应性。方法 合成两种GAD多肽 I Ag7andI Ad四聚体 ,并比较这两种四聚体阳性细胞 (tet+)的表现型及功能。结果 交叉反应性不仅表现在四聚体阳性细胞率上 ,而且在四聚体染色强度上。NOD和Balb/c来源的tet+T细胞均能被GAD抗原肽 I Ag7和I Ad四聚体交叉染色 ,并在合成的和重组的GAD抗原肽刺激下均对辐射后的NOD和Balb/c脾细胞有交叉反应。讨论 虽然I Ag7andI Ad在生物化学和生物学特性上近似 ,关键的区别在于β57上的氨基酸种类 。
