摘要
OBJECTIVE: To study the relationship between sensitivity to 5-FU and the status of a panel of microsatellite loci in three human colon cancer cell lines. METHODS: Cell viability in several concentrations of 5-FU was assessed by the MTT test. Expression of hMSH2 and hMLH1 in LoVo, SW480 and SW1116 cells were analyzed by immunocytochemical staining.Ten mononucleotide and dinucleotide microsatellite loci were analyzed by the PCR-SSLP-silver staining method. RESULTS: By MTT assay, it showed that LoVo cells were more sensitive than SW480 and SW1116 cells (0.8 micromol/L,2.2 micromol/L and 1.9 micromol/L, respectively, P
目的 我们研究了 3个人类结直肠癌细胞系中一组微卫星位点状况和 5 氟尿嘧啶 (5 FU)敏感性的相关性 ,该组位点来自或邻近于肿瘤化疗敏感性可能相关的多个因子。方法 LoVo、SW4 80和SW116细胞系对 5 FU的敏感性经MTT方法测定 ;用免疫组织化学方法检测三细胞系hMSH2、hMLH1表达水平。用PCR SSLP 银染法分别分析了三个细胞系基因组中 10个微卫星位点状况。结果 比较三个细胞系MTT分析获得的IC50 结果发现 ,对 5 FULoVo细胞系较SW4 80和SW116更加敏感 (分别为 :0 .8μmol/L ,2 .2 μmol/L和 1.9μmol/L ,P =0 .0 0 0 )。免疫组织化学检测结果显示hMSH2在SW4 80和SW1116阳性 ,百在LoVo阴性 ;hMLH1在 3个细胞系均阳性。PCR SSLP 银染法结果显示LoVo细胞系在 8/ 10个位点和其余两个细胞系不同 ,电泳条带有不同程度的增加或位移 ,而另外的 2 / 10个位点则具有相同的电泳带型。结论 该组微卫星位点和错配修复状况可以一同作为离体人类结直肠癌细胞系对 5 FU敏感性的指标 ,为了深入阐明它们能否作为临床结直肠癌化疗病人药物筛选和预后评价的有效指标 。
