南极海茸多糖咀嚼片的制备及含量测定

Preparation and determination of Durvillaea antarctica polysaccharides chewable tablets

目的研制南极海茸多糖咀嚼片的配方工艺,并建立含量测定方法。方法以南极海茸多糖为原料,采用湿法制粒压片工艺,通过正交实验,以多指标综合评分法,确定最适工艺配方。采用Gibbons比色法测定咀嚼片中南极海茸多糖的含量。结果南极海茸多糖咀嚼片的优选处方为:南极海茸多糖10%,填充剂木糖醇、甘露醇和山梨醇78%,风味剂11.5%,硬脂酸镁0.5%。采用Gibbons比色法,检测到咀嚼片中岩藻糖含量为5.90mg/g,岩藻糖在10～100μg范围内线性关系良好(r=0.999 3),回收率在95.76%～103.21%之间,RSD=1.88%(n=6)。结论按照正交优化的配方工艺,可制备出性状良好、口感酥脆、风味协调且具有海洋特色的南极海茸多糖咀嚼片。Gibbons比色法检测结果准确,操作简单快速,可用于南极海茸多糖嚼片的质量控制。

Objective To develop a novel Durvillaea antarctica polysaccharides(DAP)chewable tablet and establish its content determination method.Methods The chewable tablets were prepared by using a wet granulation method based on Durvillaea antarctica polysaccharides as raw materials.The formulation of Durvillaea antarctica polysaccharides chewable tablets was optimized by means of orthogonal experiment with a multi-index grading method.The content of Durvillaea antarctica polysaccharides was determined by Gibbons colorimetric method based on fucose.Results The optimal prescription of the chewable tablets of Durvillaea antarctica polysaccharides was as follows:Durvillaea antarctica polysaccharides 10%,the total amount of xylitol,mannitol and sorbitol was 78%,flavor agent 11.5%,and magnesium stearate 0.5%.The content of fucose in chewable tablets was determined to be 5.90 mg/g by Gibbons colorimetric method.The standard curve for fucose was found to be linear in the range of 10~100μg(r=0.999 3),The recovery was 95.76%~103.21%,RSD=1.88%(n=6).Conclusion The chewable tablets of Durvillaea antarctica polysaccharides with favorable character,crispy taste,harmonious flavor and typical of marine features were successfully obtained by means of orthogonal optimization.The Gibbons colorimetric method was proved to be accurate,simple,rapid and reproducible,and it is suitable to the content determination and quality control for the chewable tablets.