TAP68在多发性骨髓瘤患者骨髓单个核细胞中的表达及其与预后的关系

Expression of TAP68 in bone marrow mononuclear cells of patients with multiple myeloma and its relationship with prognosis

目的探讨端粒结合蛋白相关蛋白68(TAP68)在多发性骨髓瘤(MM)患者骨髓单个核细胞(BMMCs)中的表达水平及其与预后的相关性。方法选取MM患者25例作为观察组,另选取同期门诊骨髓报告为正常骨髓像的患者10例作为对照组,观察组患者采用硼替佐米联合地塞米松方案化疗。利用流式细胞术分选CD138+细胞并通过实时荧光定量检测和蛋白免疫印迹检测检测细胞中TAP68的表达水平,分析TAP68与MM临床分期的关系,并用统计学方法分析TAP68的表达量与临床检验数据的相关性。结果 TAP68在观察组BMMCs中相对表达量显著高于对照组(P<0.05);TAP68在不同分期MM患者BMMCs中表达水平差异有统计学意义(P<0.05);TAP68与M蛋白和β2微球蛋白(β2-MG)呈正相关(r=0.693、0.458,P=0.031、0.037);。治疗后,MM患者BMMCs中TAP68相对表达量显著降低(P<0.05)。结论 TAP68在MM患者BMMCs中表达量明显升高,与临床分期、M蛋白和β2-MG相关;TAP68可能在多发性骨髓瘤的发生发展中起重要作用。

Objective To investigate the expression of TAP68 in multiple myeloma and its correlation with the prognosis of patients with multiple myeloma.Methods Totally 25 patients with multiple myeloma were selected as the study group,and 10 patients with normal bone marrow were selected as the control group.The bone marrow mononuclear cells(BMMCs)were isolated from the bone marrow aspirate by Ficoll dentisy gradient centrifugation before and treatment,and bone marrow aspirate of the healthy control group was treated.The CD138+cells were purified by flow cytometry,and the mRNA level of TAP68 was detected by real time PCR,and western blot to detect the expression of TAP68.To compare the expression of TAP68 in the treatment group and the control group.To analyze the correlation between the clinical feature of TAP68 protein expression.Results Compared with the BMMCs in the normal control group,the expression of TAP68 was increased in the BMMCs in MM group.The results of western blot showed that the expression of TAP68 protein in MM group was significantly higher than that in control group.Compared with MM patients brfore theatment,the expression of TAP68 in MM patients was lower than in MM patients.The expression of TAP68 protein in MM patients was not correlated with gender,age and immunophenotyping,but was significantly correlated with ISS stage,M protein andβ2-MG.Conclusion The expression of TAP68 is closely related with the development of multiple myeloma,which may be a potential therapeutic target in tumor biotherapy.