基于AFLP标记的河八王种质资源遗传多样性分析及分子身份证构建

Genetic diversity and molecular identification card construction of Narenga porphyrocoma (Hance) Bor germplasm based on AFLP markers

以广西地区的15份河八王无性系为材料,采用AFLP标记结合毛细管电泳进行分析,计算多态性引物的总扩增条带数、多态性条带数和多态性条带比率。进行遗传相似系数和UPGMA聚类分析,并建立分子身份证。25对AFLP引物组合在15份河八王种质资源中共扩增出2 208个位点,其中多态性位点1 914个,多态性比率(PPB)为87.11%。UPGMA聚类分析表明,供试的15份河八王种质资源其遗传相似系数变化范围在0.656~0.878,在相似系数为0.706时,可划分为3个类群。结合特征带和不同引物组合方法可有效建立河八王种质资源特异分子身份证,置信概率达到99.99%。所供试河八王种质具有较丰富的遗传多样性水平,基于3对AFLP引物组合104条谱带构建的15份河八王种质分子身份证具有唯一性,AFLP标记是在分子水平上鉴定河八王种质的有效方法。

The genetic diversity of Narengaporphyrocoma(Hance)Bor germplasm collections was studied.The molecular ID card was established to provide technique support for molecular identifications,exploitation,conservation,utilization and innovation of germplasm.The genetic relationships of fifteen samples of Narengaporphyrocoma(Hance)Bor gemplasm collected from Guangxi were analyzed with AFLP(amplified fragment length polymorphism)markers and CE(capillary electrophoresis)method.The total bands,polymorphic bands and percentage of polymorphic bands(PPB)were counted.The genetic similarity was used for UPGMA(unweighted pair group method analysis)and PCA(principal component analysis).The molecular identification card of the 15 accessions of Narengaporphyroco ma(Hance)Bor gemplasm was constructed.A total of 2 208 bands were amplified with 25 pairs of AFLP primers,of which 1 914 were polymorphic with the percentage of polymorphic bands of 87.11%.The genetic similar coefficients of the 15 accessions of Narengaporphyrocoma(Hance)Bor germplasm were ranged from 0.656 to 0.878.The result of UPGMA based cluster analyses showed that these germplasm accessions were divided into three major groups at the similarity coefficient 0.706.All 15 accessions could be effectively distinguished by specific bands and different primer combinations.The specific molecular identity card of the 15 accessions of Narengaporphyrocoma(Hance)Bor germplasm was established with 99.99% probability of confidence.The unique molecular identification card of the 15 accessions of Narengaporphyrocoma(Hance)Bor germplasm was established based on the 104 bands amplified with 3AFLP primer combinations.It is indicated that the AFLP marker will be a powerful tool for identifying Narengaporphyrocoma(Hance)Bor germplasm.